Biolegend, 622602, Purified anti-NF-κB p65 Antibody, 200microl

NF-κB/p65 (nuclear factor kappa light chain enhancer in B cells p65) is a member of the Rel/dorsal family. This ubiquitously expressed nuclear protein is one subunit of the NF-kappa B complex consisting of a 65 kD transactivating subunit and a 50 kD DNA binding subunit. Both subunits are derived from larger precursor proteins. NF-κB acts as a transcriptional activator and was originally identified as an activator of kappa light chain in B cells. NF-κB/p65 is bound (with p50) to IκB inhibitor in cytoplasm in inactive form. Phosphorylation of IκB releases and NF-κB which goes to the nucleus to activate gene expression. NF-κB can be activated with LPS, TNF-α, phorbol ester, or IL-1. The Poly6226 antibody reacts with the C-terminal region of human, mouse, and rat NF-κB p65 and has been shown to be useful for Western blotting.
200microl
Verified Reactivity: Mouse, Rat, Human
Antibody Type: Polyclonal
Host Species: Rabbit
Immunogen: peptide-KLH, C-terminal
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% gelatin.Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation: The antibody was purified by antigen-affinity chromatography.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, ChIP - Verified IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 µg per mL. For immunocytochemistry, the suggested use of the reagent is a dilution range of 1:100~1:400. For ChIP applications, the suggested dilution is 1:50-1:200 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product may contain other non-IgG subtypes.
Additional Product Notes: The 50 µL size can be used for approximately 5 Western blots, and the 200 µL size can be used for approximately 20 Western blots.
Application References(PubMed link indicates BioLegend citation): Chen R, et al. 2014. J Mol Cell Cardiol. 72:263. PubMed Tan GK, et al. 2014. Acta Biomater. 10:2684. PubMed Abecassis A, et al. 2014. Cell Mol Immunol. 4:377. PubMed Sumedha N, et al. 2014. Hum Exp Toxicol. 34:506. PubMed Hua X, et al. 2015. PloS One. 10:128039. PubMed
Product Citations: Fang SY, et al. 2021. Exp Ther Med. 369:21. PubMed Smaldone G, et al. 2021. Sci Rep. 11:18237. PubMed Haddad H, et al. 2021. Int J Mol Sci. 22:. PubMed Hua X, et al. 2015. PLoS One. 10:128039. PubMed Abecassis A, et al. 2014. Cell Mol Immunol. 4:377. PubMed Chung H, et al. 2021. Nat Methods. 18:1204. PubMed Afzal SM, et al. 2020. Environ Toxicol. :. PubMed Shaker ME, et al. 2022. Saudi Pharm J. 30:291. PubMed Chaudhari S, et al. 2020. Am J Physiol Renal Physiol. 318:F1478. PubMed Ayari C, et al. 2011. J Urol. 185:1915. PubMed Ge Y, et al. 2013. J Virol. 87:11160. PubMed Miltonprabu N 2014. Hum Exp Toxicol. 34:506. PubMed Yang Z, et al. 2016. Sci Rep. 6:30263. PubMed Muri J, et al. 2020. eLife. 9:e53627.. PubMed Tortola L, et al. 2022. Mucosal Immunol. 15:656. PubMed Tan G, Tabata Y 2014. Acta Biomater. 10:2684. PubMed Chen R, et al. 2014. J Mol Cell Cardiol. 72:263. PubMed
RRID: AB_315955 (BioLegend Cat. No. 622601) AB_315956 (BioLegend Cat. No. 622602)
Structure: Member of the Rel/dorsal family. One subunit of the NF-kappa B complex consisting of a 65 kD transactivating subunit and a 50 kD DNA binding subunit. Both subunits derived from larger precursor proteins
Distribution: Ubiquitously expressed nuclear protein
Function: Transcriptional activator, originally identified as an activator of kappa light chain in B cells
Interaction: Interacts with NF-κB p50 and IκB
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Baldwin AS, et al. 1996. Annu. Rev. Immunol. 14:649 (review). 2. Chen F, et al. 1999. Clin. Chem. 45:7 (review).
Regulation: Bound (with p50) to IkB inhibitor in cytoplasm in inactive form. Phosphorylation of IkB releases and NF-kB goes to the nucleus to activate gene expression. Stimulation with LPS, TNF-α, phorbol ester, or IL-1 activates
Gene ID: 4790
UniProt: View information about NF-kappaB p65 on UniProt.org
Clone: Poly6226
Regulatory Status: RUO
Other Names: NFκB p65, nuclear factor kappa light chain enhancer in B cells p65
Isotype: Rabbit Polyclonal IgG