Biolegend, 622902, Purified anti-ATG1 (ULK1) Antibody, 100μg

ATG1/ULK1 is a Serine/Threonine protein kinase which forms a complex with the Atg13 and FIP200 proteins. This complex is the most upstream component of the core autophagy machinery, and is therefore the key initiator of autophagy in mammalian cells. AMPK positively regulates autophagy under nutrient starvation and cellular stresses by phosphorylating ULK1, leading to the activation of the autophagic process and inhibition of mTOR. mTOR plays a negative role in this process when under growth factor- and nutrient-replete conditions, as it phosphorylates ULK1 and disrupts the interaction between ULK1 and AMPK. Autophagy is a mechanism used by tumors to survive energy deprivation and chemotherapy mediated stress. Autophagy is also implicated in autoimmune (Crohn's disease) and neurodegenerative diseases. Because of its role in autophagy ULK1 is considered an attractive drug target for such diseases.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human ATG1 synthetic peptide conjugated to KLH (1-16 a.a.)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: At higher antibody concentrations, this clone may produce minor non-specific bands between 150 and 250 kD. This clone was not tested for ICC.
RRID: AB_2814476 (BioLegend Cat. No. 622901) AB_2814477 (BioLegend Cat. No. 622902)
Structure: ATG1/ULK1 is a 1050 amino acid protein with a predicted molecular weight of 112 kD.
Distribution: Cytosolic
Function: Serine/Threonine Kinase/Autophagy regulator
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Kim J, et al. 2011. Nat. Cell. Biol. 10.1038/ncb2152. Hokosawa N, et al. 2009. Mol. Biol. Cell. 20:7. Egan D, et al. 2011. Autophagy. 7:643-4.
Gene ID: 8408
UniProt: View information about ATG1 on UniProt.org
Clone: W18004A
Regulatory Status: RUO
Other Names: Serine/threonine-protein kinase ULK1, Autophagy-related protein 1 homolog, Unc-51-like kinase 1
Isotype: Rat IgG2a, κ