Biolegend, 623001, Purified anti-Gelsolin Antibody, 25μg

Gelsolin (GSN) is a calcium-regulated protein that serves actin filament assembly and disassembly and regulates cytoskeletal actin organization. GSN caps the plus end of the actin-filament  to block monomer exchange, consequently, regulating filament length near the plasma membrane. Further, it contributes to cell movement and cell morphology. Four different isoforms of the molecule have been described that are produce via alternative splicing and initiation, including a soluble form. GSN is associated with a variety of diseases such as inflammation, cancer and amyloidosis.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial human recombinant Gelsolin protein (amino acids 239-503)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2814478 (BioLegend Cat. No. 623001) AB_2814479 (BioLegend Cat. No. 623002)
Structure: GSN is a 782 amino acid protein with a predicted molecular weight of 86 kD
Distribution: Actin Filaments
Function: Functions in assembly and disassembly of actin filaments.
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Janmey, P.A. and Stossel, T.P., 1987. Nature. 325:362 Burtnick LD, et al. 1997. Cell. 90:661.
Gene ID: 2934
UniProt: View information about Gelsolin on UniProt.org
Clone: W18073A
Regulatory Status: RUO
Other Names: GSN, Gelsolin, AGEL, Actin-depolymerizing factor, ADF, Brevin
Isotype: Rat IgG2a, κ