Biolegend, 623102, Purified anti-IRF8 Antibody, 100μg

Interferon Regulatory Factor 8 (IRF8) is one of 9 members of the IRF transcription factor family that plays a critical role in the lineage commitment of myeloid cells. IRF8 has a DNA binding domain in its N-terminus region and an IRF association domain (IAD) in the C-terminus region. The IAD allows IRF8 to interact with other transcription factors and regulate gene expression. PU.1 is an IRF8 binding partner that forms a complex with IRF8 and mediates the differentiation of myeloid and lymphoid cells. The IRF8-PU.1 complex binds to a promoter-distal IRF8 binding site and induces the expression of KLF4, which drives the development of Ly6C+ monocytes. IRF8(-/-) mice are immunodeficient and highly  susceptible to infections. They are also characterized by a Chronic Myelogenous Leukemia (CML)-like syndrome marked by an increased number of myeloid progenitor cells that can only commit toward the granulocyte but not toward the macrophage lineage.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant mouse IRF8 protein (amino acids 115-424)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2814480 (BioLegend Cat. No. 623101) AB_2814481 (BioLegend Cat. No. 623102)
Structure: Mouse IRF8 is a 424 amino acid protein with a predicted molecular weight of 48.2 kD.
Distribution: Nucleus/Myeolid and lymphoid tissue
Function: Transcription factor
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Innate Immune Signaling
Antigen References: Kurotaki D, et al. 2018. Cell Reports. 22:2628. Tamura T, et al. 2005. Blood. 106:1938. Wang H, et al. 2009. Immunol. Res. 43(1-3):109.
Gene ID: 3394
UniProt: View information about IRF8 on UniProt.org
Clone: W17160D
Regulatory Status: RUO
Other Names: Interferon Regulatory Factor 8, Interferon Consensus Sequence Binding Protein 1, ICSBP, IRF-8, IMD32A, IMD32B
Isotype: Rat IgG2b, κ