Biolegend, 623401, Purified anti-Acetylated Lysine Antibody, 25μg

Proteins are reversibly and dynamically acetylated on the ε-amino group of lysine by acetyltransferases and deacetylated by deacetylases. This post-translational modification can regulate protein function (interactions with other proteins and DNA binding). Histones and transcription factors (PCAF, p53, p300, etc) appear to be the major targets of acetyltransferases. Acetylation is usually associated with chromatin remodeling and transcriptional activation, although in some cases (telomeres) it is associated with gene silencing. The 15G10 antibody recognizes acetylated lysine residues on proteins.
25μg
Verified Reactivity: Species independent
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Acetylated protein mixture
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. For immunocytochemistry: Use a dilution range of 1-4 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Kuo HP, et al. 2010. Sci Signal. 3:Ra9. PubMed
Product Citations: Yang JM, et al. 2022. Sci Adv. 8:eabo0183. PubMed Zhang W, et al. 2022. J Clin Invest. 132: . PubMed Sun X, et al. 2022. J Am Heart Assoc. 11:e025266. PubMed Lee J, Homma T, Fujii J, et al. 2017. Toxicol Lett. 10.1016/j.toxlet.2017.06.005. PubMed Wu Q, et al. 2021. J Immunother Cancer. 9: . PubMed Kuo H, et al. 2010. Sci Signal. 3:ra9. PubMed Chen G, et al. 2019. EMBO Mol Med. 11:e10469. PubMed Han S, et al. 2017. Nat Commun. 8:14568. PubMed
RRID: AB_315967 (BioLegend Cat. No. 623401) AB_315968 (BioLegend Cat. No. 623402)
Structure: Proteins are acetylated by the reversible transfer of acetyl-CoA to the ε-amino group of lysine
Function: Post-translational modification of proteins by acetyltransferases and deacetylated by deacetylases to regulate protein-protein and protein-DNA interactions
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics
Antigen References: 1. Wang S, et al. 2004. Novartis Found. Symp. 259:238. 2. Legube G, et al. 2003. EMBO Rep. 4:944. 3. Chen LF, et al. 2003. J. Mol. Med. 81:549.
Gene ID: NA
Clone: 15G10
Regulatory Status: RUO
Other Names: Proteins are acetylated by the reversible transfer of acetyl-CoA to the ε-amino group of lysine
Isotype: Mouse IgG2b, κ