Biolegend, 623405, Alexa Fluor® 647 anti-Acetylated Lysine Antibody, 25tests

Proteins are reversibly and dynamically acetylated on the ε-amino group of lysine by acetyltransferases and deacetylated by deacetylases. This post-translational modification can regulate protein function (interactions with other proteins and DNA binding). Histones and transcription factors (PCAF, p53, p300, etc) appear to be the major targets of acetyltransferases. Acetylation is usually associated with chromatin remodeling and transcriptional activation, although in some cases (telomeres) it is associated with gene silencing. The 15G10 antibody recognizes acetylated lysine residues on proteins.
25tests
Verified Reactivity: Species independent
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Acetylated protein mixture
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Kuo HP, et al. 2010. Sci Signal. 3:Ra9. PubMed
Product Citations: Martins VF, et al. 2019. Am J Physiol Cell Physiol. 317:C964. PubMed Martins VF, et al. 2021. JCI Insight. Online ahead of print. PubMed Masuda N, et al. 2021. BMC Cancer. 21:1269. PubMed
RRID: AB_2565505 (BioLegend Cat. No. 623405) AB_2565506 (BioLegend Cat. No. 623406)
Structure: Proteins are acetylated by the reversible transfer of acetyl-CoA to the ε-amino group of lysine
Function: Post-translational modification of proteins by acetyltransferases and deacetylated by deacetylases to regulate protein-protein and protein-DNA interactions
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics
Antigen References: 1. Wang S, et al. 2004. Novartis Found. Symp. 259:238. 2. Legube G, et al. 2003. EMBO Rep. 4:944. 3. Chen LF, et al. 2003. J. Mol. Med. 81:549.
Gene ID: NA
Clone: 15G10
Regulatory Status: RUO
Isotype: Mouse IgG2b, κ