Biolegend, 623851, Purified anti-Neu1 Antibody, 25μg

Neu1, a member of the sialidase enzyme family, plays a crucial role in cellular processes by removing sialic acid (N-acetylneuraminic acid) residues from glycoproteins and glycolipids on the cell surface. This process, known as desialylation, impacts various cellular functions, including cell-cell interactions, signal transduction, and immune responses. Additionally, desialylation recycles sialic acid, contributing to the maintenance of cellular sialic acid levels critical for normal physiological processes. Alterations in Neu1 activity leads to sialidosis, a lysosomal storage disease resulting in an accumulation of sugar-protein complexes.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C-terminal fragment of recombinant human Neu1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody (clone A18167C) is not suitable for Western blot (WB) or immunocytochemistry (ICC). For immunohistochemistry (IHC), we recommend antigen retrieval with Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0). Antigen retrieval with Sodium Citrate is not recommended.
RRID: AB_3097496 (BioLegend Cat. No. 623851) AB_3097496 (BioLegend Cat. No. 623852)
Structure: Neu1 is a 415 amino acid protein with a predicted molecular weight of 45.5 kD.
Distribution: Cytoplasmic, lysosomal
Function: Sialidase, with a preference for alpha 2-3 and alpha 2-6 sialyl linkage
Molecular Family: Cytokines/Chemokines, Enzymes and Regulators
Antigen References: Khan A & Sergi CM. 2022. Front Pharmacol. 13:902259. Seyrantepe V, et al. 2003. Hum Mutat. 22:343-52 Toussaint K, et al. 2022. Cancers. 14:4868.
Gene ID: 4758
UniProt: View information about Neu1 on UniProt.org
Clone: A18167C
Regulatory Status: RUO
Other Names: Neuraminidase 1 (NEU1), sialidase 1 (SIAL1), sialidase-1 Acetylneuraminyl hydrolase (NANH), G9 sialidase, Lysosomal sialidase, N-acetyl-alpha-neuraminidase 1
Isotype: Mouse IgG2a, κ