Biolegend, 623902, Purified anti-LAT Antibody, 100μg

LAT (linker for activation of T-cells) is a 36 kD phospho-tyrosine adaptor protein containing SH2 domains and a CVRC motif. Two LAT isoforms have been reported with approximate molecular weights of 36 kD and 38 kD. LAT is localized to lipid rafts, and is also a potential type III transmembrane protein, thought to be expressed in the juxtanuclear intracellular compartment. LAT links TCR engagement to Ras-MAPK activation, Ca 2+ flux, IL-2 expression and is required for T-cell development. LAT is phosphorylated when activated by TCR cross-linking, where it recruits signaling molecules. LAT activation can be prevented by polyunsaturated fatty acids. LAT is modified by ZAP-70 and Syk phosphorylation and can also be palmitoylated. LAT has been reported to be associated with Grb2, Gads, Grap, PLC-γ1, p85 subunit PI3K, ZAP-70, Syk, Itk, Vav, SLP-76, and cbl.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial recombinant protein (cytoplasmic domain of human LAT)
Formulation: This LAT antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store between 2°C and 8°C.
Application: WB - Quality tested ICFC - Verified IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The LAT1111 monoclonal antibody recognizes the cytoplasmic domain of human LAT and has been shown to be useful for Western blotting and immunoprecipitation. The LAT1111 antibody does not cross-react with mouse LAT.
Product Citations: Ghosh S, et al. 2020. Cell Reports. 30(10):3434-3447. PubMed
RRID: AB_2133119 (BioLegend Cat. No. 623901) AB_2133118 (BioLegend Cat. No. 623902)
Structure: SH2 domains, CVRC motif; two isoforms 36 kD, 38 kD
Distribution: Lipid rafts, potential type III transmembrane protein, juxtanuclear intracellular compartment
Function: Links TCR engagement to Ras-MAPK activation, Ca2+ flux, IL-2 expression; required for T-cell development
Interaction: Grb2, Gads, Grap, PLC-γ1, p85 subunit PI3K, ZAP-70, Syk, Itk, Vav, SLP-76, cbl
Modification: Phosphorylation by ZAP-70, Syk, Palmitoylated
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: Phospho-Proteins
Antigen References: 1. Zhang W, et al. 1998. Cell. 92:83. 2. Zeyda M, et al. 2002. J. Biol. Chem. 277:28418. 3. Perez-Villar J, et al. 2002 Biochemistry. 41:10732. 4. Hartgroves L, et al. 2003 J. Biol. Chem. 278:20389.
Regulation: Phosphorylated when activated by TCR cross-linking, recruits signaling molecules, activation prevented by polyunsaturated fatty acids
Gene ID: 27040
UniProt: View information about LAT on UniProt.org
Clone: LAT1111
Regulatory Status: RUO
Other Names: Linker for activation of T-cells, 36 kD phospho-tyrosine adaptor protein
Isotype: Mouse IgG1, κ