Biolegend, 624151, Purified anti-CD326 (EpCAM) Antibody, 25μg

CD326 is also known as EpCAM (Epithelial cell adhesion molecule), a glycosylated I transmembrane protein containing six disulfide bridges and one THYRO domain. It is highly expressed in bone marrow, colon, lung, and most normal epithelial cells where it functions as an adhesion molecule. In addition to cell adhesion, CD326 is also involved in cellular signaling, cell migration, proliferation, and differentiation. Recently, it has been reported that CD326 expression occurs during the early steps of erythrogenesis. CD326 functions as a homotypic calcium-independent cell adhesion molecule and is involved in carcinogenesis via its ability to induce genes c-myc, e-fabp, and cyclins A & E involved in cellular metabolism and proliferation. Research studies have demonstrated that CD326 plays a role in tumorigenesis and metastasis of carcinomas, therefore, has the potential to serve as a prognostic marker and an immunotherapeutic target.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Extracellular domain of recombinant human CD326 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone does not cross-react with mouse. For immunocytochemistry (ICC), we recommend using ice-cold methanol fixation. For immunohistochemistry (IHC-P), the suggested antigen retrieval buffer is citrate buffer (Ca. No. 420901) or Tris-EDTA pH 9.0.
RRID: AB_3097236 (BioLegend Cat. No. 624151) AB_3097236 (BioLegend Cat. No. 624152)
Structure: CD326 is an 314 amino acid protein with a predicted molecular weight of 35 kD
Distribution: Highly expressed in bone marrow, colon, lung, and most normal epithelial cells. Also highly expressed on carcinomas of gastrointestinal origin. Expressed during early erythrogenesis.
Function: Homotypic calcium-independent cell adhesion. CD326 is involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation.
Interaction: Monomer. Interacts with phosphorylated CLDN7
Ligand/Receptor: LAIR-1 (CD305), LAIR-2 (CD306), Ep-CAM
Cell Type: Epithelial cells
Biology Area: Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Strnad J, et al. 1989. Cancer Res. 49:314. Munz M, et al. 2004. Oncogene. 23:5748. Maetzel D, et al. 2009. Nature Cell Biology. 11:162–71. Osta WA, et al. 2004. Cancer Res. 64:5818–24. Litvinov SV, et al. 1996. Journal of Pathology. 148:865–75. Armstrong A, et al. 1996. Cancer Biology & Therapy. 2:320–6. Rao CG, et al. 2005. Int J Oncol. 27:49.
Gene ID: 4072
UniProt: View information about CD326 on UniProt.org
Clone: W21090A
Regulatory Status: RUO
Other Names: Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, TROP1
Isotype: Rat IgG2a, κ