Biolegend, 624351, Purified anti-MAVS Antibody, 25μg

Mitochondrial antiviral signaling protein (MAVS) is essential and specific for innate immunity by activating IRF3, IRF7 and NF-κB in response to viral infection. MAVS is located on the outer membranes of mitochondria, peroxisomes, and mitochondria-associated endoplasmic reticulum membranes (MAM). The MAVS protein contains an N-terminal CARD domain and a C-terminal mitochondrial transmembrane domain. MAVS acts downstream of the RIG-I RNA helicase and sensing viral RNA, leading to the recruitment of IKKε, TRIF and TRAF6. Some viruses have developed strategies to evade these innate defense mechanisms by using proteases that cleave MAVS and prevent its localization to mitochondria. The C-terminal region of MAVS cleavage by the hepatitis C virus (HCV) protease allows HCV to evade the host immune system.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial Recombinant human MAVS protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The clone does not cross-react with mouse in western blotting (WB). For immunocytochemistry (ICC), we recommend to use 4% PFA fixation followed by permeabilization with 0.5% Triton X-100 or ice cold-methanol, or ice-cold methanol fixation. For immunohistochemistry (IHC-P), antigen retrieval with Citrate Buffer (Cat. No. 420901) or Tris-EDTA pH 9.0 is recommended.
RRID: AB_3097498 (BioLegend Cat. No. 624351) AB_3097498 (BioLegend Cat. No. 624352)
Structure: MAVS protein is a 540 amino acid protein with a predicted molecular weight of 57 kD
Distribution: Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes
Function: Innate immunity
Interaction: RIGI, IFIH1/MDA5, TRAF2, TRAF6, C1QBP, FADD, RIPK1, CHUK, IKBKB and NLRX1
Cell Type: Epithelial cells, Leukocytes, Monocytes, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity
Molecular Family: Innate Immune Signaling
Antigen References: Seth RB, et al. 2005. Cell. 122:669-82. Sun Q, et al. 2006. Immunity. 24:633-42. Xu LG, et al. 2005. Mol Cell. 19: 727-40. Yoneyama M, et al. 2004. Nat Immunol. 5:730-7. Lin R, et al. 2006. J Virol. 80:6072-83. Chen Z, et al. 2007. J Virol. 81:964-76. Li XD, et al. 2005. PNAS. 102:17717-22.
Gene ID: 57506
UniProt: View information about MAVS on UniProt.org
Clone: W21144C
Regulatory Status: RUO
Other Names: Interferon beta promoter stimulator protein 1, IPS-1, Virus-induced-signaling adapter, VISA, Mitochondrial Antiviral-Signaling Protein
Isotype: Rat IgG2b, κ