Biolegend, 624652, Purified anti-Serpin A12 Antibody, 100μg

Human Serpin A12, also known as Vaspin, is a secreted adipokine that is found in visceral adipose tissue. It belongs to the serpin superfamily, class A. It's known protease target is KLK7, a member of the kallikrein family. In humans, Serpin A12 expression may increase at the onset of diabetes and decrease when the disease aggravates. The administration of recombinant Serpin A12 in a diet-induced mouse obesity model suggests that it may represent an insulin-sensitizing adipokine. Low serum concentration of Serpin A12 may be a risk factor for the progression of type-2 diabetes mellitus. Although its mechanism of action is not fully understood, KLK7 inhibition seems to be the most likely underlying physiological mechanism for the compensatory effects on obesity-induced insulin resistance. In addition to its relation to diabetes, Serpin A12 exerts protective effects on heart disease. Its low concentrations correlate with coronary artery diseases’ severity and unstable angina pectoris. Serpin A12 may inhibit endothelial cell apoptosis, attenuate high glucose stimulated vascular smooth muscle cell proliferation, and chemokinesis. It also acts as a ligand for the cell surface GRP78/voltage dependent anion channel complex in endothelial cells and promotes proliferation, inhibits apoptosis, and protects against diabetes mellitus associated vascular injuries.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant fragment of human Serpin A12
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For immunohistochemistry, Citrate buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 is recommended.
RRID: AB_3083413 (BioLegend Cat. No. 624651) AB_3083413 (BioLegend Cat. No. 624652)
Structure: Monomer
Distribution: Expressed in visceral adipose tissues and liver. Possibly present in adipocytes. Present in the plasma and serum
Function: Adipokines that modulate insulin action by specifically inhibiting target protease KLK7 in white adipose tissues
Interaction: KLK7, GRP78/VDAC complex
Biology Area: Cardiovascular Biology, Signal Transduction
Antigen References: Li HL, et al. 2011. Clin Chem Lab Med. 49:1547-54. Jung CH, et al. 2011. Biochem. Biophys. Res. Commun. 413:264-9. Li H, et al. 2013. Atherosclerosis. 228:61-8. Nakatsuka A, et al. 2012. Diabetes. 61:2823-32. Heiker JT, et al. 2013. Cell Mol. Life Sci. 70:2569-83. Hida K, et al. 2005. Proc. Natl. Acad. Sci. USA. 30:10610. Jian W, et al. 2014. PLoS One. 9:e94763.
Gene ID: 145264
UniProt: View information about Serpin A12 on UniProt.org
Clone: A16088B
Regulatory Status: RUO
Other Names: Vaspin, Visceral adipose tissue-derived serine protease inhibitor, Visceral adipose-specific serpin, OL-64
Isotype: Mouse IgG2b, κ