Biolegend, 625001, Purified anti-SLP-76 Antibody, 50microl

SLP-76 is a human Src homology domain-containing leukocyte protein. This cytoplasmic adaptor phosphoprotein contains both SH2 and SAM domains and is involved in B and T cell receptor signaling. The amino terminus of this protein contains three 17 amino acid repeats with conserved tyrosine and acidic residues (DYE(S/P)P) as well as a proline rich region and is known to associate with many proteins involved in T and B cell signaling including Grb2, LAT, Vav1, SLAP-130, SHP-1, and phospholipase C gamma 2. SLP-76 can be phosphorylated on multiple tyrosine residues by the upstream kinases ZAP-70 and Lck. SLP-76 phosphorylation plays an important role in T cell-mediated IL-2 production by allowing phosphorylated Vav to bind; this complex (SLP-76/Vav) stimulates NF-AT and IL-2 gene activation after TCR engagement. Overexpression of SLP-76 has been shown to result in enhanced IL-2 transcription after TCR signaling. The H76 monoclonal antibody recognizes human SLP-76 and has been shown to be useful for Western blotting.
50microl
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: amino acids 216-434 of human SLP-76
Formulation: This antibody is provided in phosphate-buffered solution, containing 0.09% sodium azide.Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation: The antibody was purified by affinity chromatography.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: Upon receipt, aliquot and store frozen at -20°C. Avoid freeze-thaw cycles.
Application: WB - Quality tested IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1:1000 dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: The 200 µL size can be used for approximately 40 Western blots.
Application References(PubMed link indicates BioLegend citation): Deford-Watts LM, et al. 2011. J. Immunol. 186:6839. PubMed.
Product Citations: Bacchelli C, et al. 2017. J Allergy Clin Immunol. 139:634. PubMed DeFord-Watts L, et al. 2011. J Immunol. 186:6839. PubMed
RRID: AB_2136888 (BioLegend Cat. No. 625002)
Structure: Phosphoprotein 76 kD containing SH2 domain in the C-terminus
Distribution: Expressed in leukocytes (monocytes, T and B cells), thymus, spleen, placenta, and lung.
Function: Adaptor molecule involved in B cell receptor and T cell receptor signaling
Interaction: Grb2, LAT, Vav1, SLAP-130, SHP-1, phospholipase C gamma 2
Modification: Phosphorylation (Y113, Y128, Y145, Y423, Y426)
Cell Type: B cells, Monocytes, T cells
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: 1. Jackman KJ, et al. 1995. J. Biol. Chem. 270:7029. 2. Wardenberg JB, et al. 1996. J. Biol. Chem. 271:19641. 3. Tuosto L, et al. 1996. J. Exp. Med. 184:1161. 4. Motto DG, et al. 1996. J. Exp. Med. 183:1937.
Gene ID: 3937
UniProt: View information about SLP-76 on UniProt.org
Clone: H76
Regulatory Status: RUO
Other Names: Lymphocyte cytoplasmic protein 2 (LCP2), SH2 domain containing leukocyte 76kD protein
Isotype: Mouse IgG2a, κ