Biolegend, 625052, Purified anti-B-Raf Antibody, 100μg

B-Raf is one of three proteins in the RAF family of serine/threonine protein kinases. B-Raf regulates the mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) pathway. Like the other two RAF kinases, A-Raf and C-Raf, B-Raf relays extracellular signals to the nucleus through the MAPK/ERK pathway and regulates cell division, differentiation, and secretion. RAF proteins share similar protein domains and motifs, including multiple protein binding domains such as Ras, lipid, and the 14-3-3 binding motif. RAF proteins mainly reside in the cytoplasm in an inactive state by binding to 14-3-3 proteins. RAFs translocate to the membrane compartment by activation and binding with RAS proteins. Similar to other RAF kinases, B-Raf phosphorylates MAP2K1 (MEK) proteins to activate the MAPK/ERK pathway. Mutations in the gene which encodes B-Raf are highly correlated with inherited cardiofaciocautaneous syndrome and various types of cancers. The B-Raf V600E mutation results in a constitutively active kinase leading to aberrant cell proliferation and invasion. Such mutations are linked to hairy cell leukemia, melanoma, colon cancer, and non-small-cell lung cancer. B-Raf inhibitors are considered as anticancer targets for metastatic melanoma, kidney cancer, and liver cancer.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human B-Raf
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product cross-reacts with mouse in western blotting (WB). This product is not recommended for immunocytochemistry (ICC).
RRID: AB_3097499 (BioLegend Cat. No. 625051) AB_3097499 (BioLegend Cat. No. 625052)
Structure: It forms homodimers and heterodimers with C-Raf. It binds to 14-3-3, Ras, or MAP2K1.
Distribution: Cytoplasm, membrane, and nucleus
Function: Phosphorylates MAP2K1 and activates the MAPK/ERK pathway
Interaction: Regulates the MAPK and ERK pathways; interacts with PFKFB2
Biology Area: Cancer Biomarkers, Cardiovascular Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability, Neuroscience, Synaptic Biology
Molecular Family: Postsynaptic proteins, Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References: Davies H, et al. 2002. Nature. 17(6892):949-54. Wajapeyee N, et al. 2008. Cell. 132:363-74. Tiacci E, et al. 2011. N Engl J Med. 364:2305-15. Dankner M, et al. 2018. Oncogene. 37:3183-3199.
Gene ID: 673
UniProt: View information about B-Raf on UniProt.org
Clone: W21141A
Regulatory Status: RUO
Other Names: BRAF, V-Raf Murine Sarcoma Viral Oncogene Homolog B1, Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, Proto-oncogene B-Raf, BRAF1, BRAF-1, RAFB1
Isotype: Rat IgG2b, κ