Biolegend, 625602, Purified anti-PLZF Antibody, 100μg

Promyelocytic leukaemia zinc finger protein (PLZF) is a transcription factor that belongs to the POK (POZ and Krüppel) family of transcriptional repressors. It is involved in cell cycle control, and plays an essential role in limb development, differentiation of myeloid cells, and spermatogenesis. In certain acute myeloid leukemias, PLZF exists as a translocation product with Retinoic Acid Receptor-α (PLZF-RARα) that has a dominant negative effect on hematopoietic differentiation and is capable of activating Wnt signaling by upregulating the γ-catenin promoter. PLZF can also repress transcription of TP53 and also destabilizes p53 protein through enhanced ubiquitination.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human PLZF protein corresponding to amino acid residues 1-254
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone has not been tested for ICC or IP.
RRID: AB_2814490 (BioLegend Cat. No. 625601) AB_2814491 (BioLegend Cat. No. 625602)
Structure: PLZF is a 673 amino acid protein with a predicted molecular weight of 74 kD.
Distribution: Nucleus/widely expressed
Function: Transcriptional repressor/epigenetic regulator
Cell Type: Mesenchymal Stem Cells, NKT cells, T cells
Biology Area: Cell Biology, Immunology, Signal Transduction, Stem Cells, Transcription Factors
Antigen References: Choi WI, et al. 2014. J. Biol. Chem. 289: 18625. Constantinides MG, et al. 2011. J. Immunol. 187:309. Kovalovsky D, et al. 2008. Nat. Immunol. 9:1055. Zheng X, et al. 2004. Blood. 103:3535. Chen Z, et al. 1994. Proc. Natl. Acad. Sci. USA 91:1178. Chen Z, et al. 1993. EMBO J. 12:1161.
Gene ID: 7704
UniProt: View information about PLZF on UniProt.org
Clone: W18031A
Regulatory Status: RUO
Other Names: ZBTB16, Zinc Finger And BTB Domain Containing 16, ZNF145, Promyelocytic Leukaemia Zinc Finger
Isotype: Rat IgG2b, λ