Biolegend, 625702, Purified anti-Oct-1 (POU2F1) Antibody, 100μg

Oct-1 is a member of the Pit-Oct-Unc (POU) domain family of transcription factors, which are characterized by a bipartite POU domain. The POU domain that contains two DNA-binding subdomains; an N-terminal POU-specific subdomain and a C-terminal homeobox subdomain, each of which are tethered by a variable linker sequence. Oct-1 can function as both a transcriptional activator and repressor, and controls the expression of housekeeping genes such as those encoding Histone H2B and snRNPs, as well as genes involved in development and differentiation. The highly-modular POU domain enables Oct-1 to drive diverse transcriptional programming that occurs in a tissue-specific manner. The POU domains confers the flexibility for Oct-1 to function as both a monomer, dimer, and heterodimer, and also facilitates the interaction between Oct-1 with a broad set of transcriptional cofactor proteins. Moreover, Oct-1 undergoes multiple post-translational modifications that confers DNA-binding specificity to certain promoters, including phosphorylation, ubiquitination, and sumoylation. Oct-1 is highly-expressed in many types of cancers, and functionally different isoforms produced from alternative promoter usage have been reported.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant protein corresponding to human OCT-1 amino acid residues 251-500
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was not tested for ICC Non-specific band present in Ramos at 60 kD
RRID: AB_2814492 (BioLegend Cat. No. 625701) AB_2814493 (BioLegend Cat. No. 625702)
Structure: Oct-1 is a 743 amino acid protein with a predicted molecular weight of 76kD
Distribution: Nucleoplasm/Ubiquitously expressed
Function: Transcription factor
Biology Area: Cell Biology, Transcription Factors
Antigen References: Pankratova EV, et al. 2018. Oncotarget. 9:29892 Strum RA and Herr W. et al. 1988. Nature. 336:601. Strum RA, et al. 1988. Genes Dev. 2:1582.
Gene ID: 5451
UniProt: View information about Oct-1 on UniProt.org
Clone: W18054D
Regulatory Status: RUO
Other Names: POU domain, class 2, transcription factor 1, Octamer-binding transcription factor 1, NF-A1
Isotype: Rat IgG2a, κ