Biolegend, 626152, Purified anti-Parvalbumin Antibody, 100μg

Parvalbumin is a calcium-binding protein with diverse functions in various tissues. Originally identified in skeletal muscle fibers, parvalbumin holds particular significance in the central nervous system. In neurons, parvalbumin acts as a calcium buffer, helping to maintain precise control over calcium ion levels within the cell. This function is critical for regulating synaptic transmission, especially at inhibitory synapses where it influences the release of the neurotransmitter GABA to synchronize the activity of neural networks. Additionally, parvalbumin contributes to the plasticity of synaptic connections, affecting learning and memory. Overall, parvalbumin's multifaceted role underscores its importance in shaping the intricate dynamics of the nervous system and maintaining its proper functioning.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant N-terminus fragment of human parvalbumin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For IHC-P in mouse tissues, we recommend antigen retrieval with Citrate Buffer, 10X (Cat. No. 420901). Clone A17130G is not suitable for Western Blot. Clone A17130G is not suitable for ICC. Clone A17130G is not suitable for IHC-P in human tissues. Although signal is detected, signal strength is low.
RRID: AB_3097265 (BioLegend Cat. No. 626151) AB_3097265 (BioLegend Cat. No. 626152)
Structure: Parvalbumin is a 110 amino acid protein with a predicted molecular weight of 12.1 kD
Distribution: Cytoplasmic; Inhibitory neurons, GABA-ergic neurons
Function: Calcium regulation
Cell Type: GABAergic Neurons, Mature Neurons, Neurons
Biology Area: Signal Transduction
Antigen References: Permyakov EA, et al. 2017. Curr Protein Pept Sci. 18:780-794. Rupert DD and Shea SD. 2022. Front Neural Circuits. 16:886629. Hu H, et al. 2014. Science. 345:1255263. Juarez P and Martinez Cerdeno V. 2022. Front Psychiatry. 13:913550.
Gene ID: 5816
UniProt: View information about Parvalbumin on UniProt.org
Clone: A17130G
Regulatory Status: RUO
Other Names: Parvalbumin alpha (PVALB), D22S749
Isotype: Mouse IgG2b, κ