Biolegend, 626352, Purified anti-Nestin Antibody, 200microl

Nestin is a class VI intermediate filament protein originally found in neuronal stem cells, and is required for survival and renewal of neural progenitor cells. In mixtures, Nestin forms heterodimers with vimentin or internexin, participating in the remodeling of the cell. Nestin was found to be expressed on vascular endothelial progenitor cells and various neoplasms. It is proposed to be a novel angiogenesis marker and cancer therapeutic target.
200microl
Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Rabbit
Immunogen: Recombinant fragment of human nestin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICC - Quality tested WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a dilution range of 1:50 - 1:1000 is recommended. For western blotting, the suggested dilution range of this reagent is 1:5000 - 1:50000. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For Immunocytochemistry, either 4% paraformaldehyde/Triton X-100, 4% paraformaldehyde/methanol, or methanol only is recommended for fixation/permeabilization.
RRID: AB_3097319 (BioLegend Cat. No. 626351) AB_3097319 (BioLegend Cat. No. 626352)
Structure: 1621 amino acids with predicted molecular weight of 177 kD; the observed molecular weight is larger than 250 kD
Distribution: Cytosol/Neural stem cells and various cancer cells
Function: Required for survival and renewal of neural progenitor cells; plays roles in angiogenesis and tumor formation
Interaction: Vimentin, alpha-internexin and FHOD3
Cell Type: Neural Stem Cells
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Antigen References: Matsuda Y, et al. 2013. World J Gastroenterol. 19:42-8. Matsuda Y, et al. 2012. Med Mol Morphol. 45:59-65. Krupkova O Jr, et al. 2010. Neoplasma. 57:291-8. Hombach-Klonisch S, et al. 2008. Arch Immunol Ther Exp (Warsz). 56:165-80. Pilkington GJ. 2005. Cell Prolif. 38:423-33.
Gene ID: 10763
UniProt: View information about Nestin on UniProt.org
Clone: Poly22A17
Regulatory Status: RUO
Other Names: Nes
Isotype: Rabbit Polyclonal IgG