Biolegend, 626453, Alexa Fluor® 647 anti-PTEN Phospho (Ser380) Antibody, 25μg

PTEN is a tumor suppressor gene which encodes a phosphatase that preferentially dephosphorylates phosphoinositide substrates and thereby modulates cell cycle progression and cell survival. PTEN is an important negative regulator of the PI3K/AKT/mTOR signaling pathway by dephosphorylating PIP3. PTEN is commonly mutated in human cancer, hence, loss of expression/function of PTEN due to mutations have been observed in prostate cancer, glioblastoma, endometrial, lung and breast cancers. Other non-cancerous clinical disorders associated with PTEN mutation are Cowden syndrome, Bannayan-Riley-Ruvalcaba syndrome, and proteus-like syndrome. PTEN is phosphorylated at several residues and some lead to increase in activity while others lead to loss of function and degradation. Specifically, phosphorylation at serine 380 residue by casein kinase 2 (CK2), leads to degradation of PTEN in the proteasome.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide from human PTEN phosphorylated at Serine 280
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: For use of this antibody (clone A22005D) in intracellular staining in flow cytometry (ICFC), we recommend using True-Phos ™ Perm Buffer (Cat. No. 425401) for fixation/permeabilization. For use of this antibody (clone A22005D) in immunohistochemistry on FFPE tissues (IHC-P), we recommend antigen retrieval with Tris-EDTA pH 9.0 Buffer (Cat. No. 422703).
RRID: AB_3662411 (BioLegend Cat. No. 626453)
Structure: PTEN is a 403 amino acid protein with a predicted molecular weight of 55 kD. It has a tyrosine phosphatase domain and a C2 domain
Distribution: Cytoplasmic (Inner cellular membrane) and nuclear
Function: PTEN functions as a lipid and protein phosphatase. It is negatively regulated by phosphorylation at the C-terminal domain which leads to proteasomal degradation.
Interaction: P53, PTK2, MAGI3, CSNK2A2, NEDD4, NR3C4, MVP
Biology Area: Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Signal Transduction, Transcription Factors
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Dempsey DR, et al. 2021. Nature Structural & Molecular Biology. 28:858-868. Gupta A, et al. 2016. JBC. 291:18465-73. Song MS, et al. 2012. Nature Reviews Molecular Cell Biology. 13:283-96. Vazquez F, et al. 2000. Mol Cell Biol. 20:5010-8.
Gene ID: 5728
UniProt: View information about PTEN Phospho Ser380 on UniProt.org
Clone: A22005D
Regulatory Status: RUO
Other Names: Phosphatase and tensin homolog, PTEN1, BZS, DEC, CWS1, GLM2, MHAM, TEP1, MMAC1
Isotype: Mouse IgG1, κ