Biolegend, 626810, Alexa Fluor® 647 anti-c-Myc Antibody, 100μg

The 9E10 monoclonal antibody recognizes the amino acid sequence EQKLISEEDL, which is a specific portion of the human c-myc gene product. This antibody is highly specific and is unlikely to alter the activity of the cloned sequence.
100μg
Verified Reactivity: Epitope tag
Reported Reactivity: Species independent
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Amino acids 408-439, C-terminal region of human c-myc
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: *Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody. Reactivity with endogenous myc in applications other than IHC-P (ICC, WB, etc.) is not verified.
Application References(PubMed link indicates BioLegend citation): Rao H, et al. 2001. Nature. 410:955. Peters CS, et al. 2001. J Biol Chem. 276:13718. Munro S, Pelham HR. 1987. Cell. 48:899. Evan GI, et al. 1985. Mol Cell Biol. 5:3610.
Product Citations: Merljak E, et al. 2023. Nat Commun. 14:1995. PubMed Gioulbasani M, et al. 2024. Commun Biol. 1606:7. PubMed
RRID: AB_2888732 (BioLegend Cat. No. 626809) AB_2888732 (BioLegend Cat. No. 626810)
Biology Area: Cell Biology, Protein Purification, Protein Synthesis
Antigen References: Adams JM, et al. 1983. Proc. Natl. Acad. Sci. USA 80:1982. Atchley WR, et al. 1995. Proc. Natl. Acad. Sci. USA 92:10217. Battey J, et al. 1983. Cell 34:779. Beimling P, et al. 1985. Biochemistry 24:6349.
Gene ID: 4609
UniProt: View information about c-Myc on UniProt.org
Clone: 9E10
Regulatory Status: RUO
Other Names: myc tag, c-Myc tag, cMyc tag, Myc epitope tag
Isotype: Mouse IgG1, κ