Biolegend, 627001, Purified anti-GRB2 Antibody, 25μg

GRB2 is a receptor tyrosine kinase (RTK) adaptor protein that transduces signaling cascades from activated receptors. Upon receptor stimulation, GRB2 binds phosphorylated RTK tyrosine residues via a central SH2 domain; flanking GRB2 SH3 domains facilitate the recruitment of downstream effectors to the activated RTK.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Synthetic peptide corresponding to amino acid residues 199-214 of human GRB2.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone has not been tested for ICC or IP.
RRID: AB_2810687 (BioLegend Cat. No. 627001) AB_2810688 (BioLegend Cat. No. 627002)
Structure: GRB2 is a 217 amino acid protein with a predicted molecular weight of 25 kD.
Distribution: Plasma membrane-associated/ubiquitously expressed
Function: Adaptor protein in RTK signaling pathways
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Adaptor Proteins, Phospho-Proteins
Antigen References: Lowenstein EJ, et al. 1992. Cell. 70:431-42. Matuoka K, et al. 1992. Proc Natl Acad Sci U S A. 89:9015-9. Skolnik EY, et al. 1993. Science. 260:1953-5. Pendergast AM, et al. 1993. Cell. 75:175-85.
Gene ID: 2885
UniProt: View information about GRB2 on UniProt.org
Clone: W17230A
Regulatory Status: RUO
Other Names: Growth Factor Receptor Bound Protein 2, SH2/SH3 Adaptor Protein GRB2, ASH, Epidermal Growth Factor Receptor-Binding Protein GRB2, Abundant SRC Homology, MSTP084, Grb3-3, MST084, NCKAP2
Isotype: Rat IgG2b, κ