Biolegend, 627502, Purified anti-VEGF Antibody, 200microl

VEGF, also known as vascular endothelial growth factor, vasculotropin, and vascular permeability factor, is a widely expressed mitogen for vascular endothelial cells that has been shown to promote angiogenesis and increase permeability of capillary blood vessels. VEGF is a member of the platelet-derived growth factor (PDGF) family and is produced as a homodimeric protein with approximate molecular weights of 34-46 kD. The variance in molecular weight is a result of alternative splicing events that encode monomeric proteins having 121, 165, 189 or 206 amino acids. VEGF121 and VEGF165 are secreted proteins whereas VEGF189 and VEGF206 are strongly cell-associated. VEGF binds to VEGF-R1 (also known as Flt-1) and VEGF-R2 (also known as KDR). Inhibition of VEGF biding to VEGF-Rs has been the focus of a number of tumor therapeutic strategies. The Poly6275 antibody recognizes the N-terminal region of human VEGF has been shown to be useful for Western blotting of both reduced and non-reduced VEGF.
200microl
Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Rabbit
Immunogen: Peptide mapping to a domain near the N-terminus
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% gelatin.Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation: The antibody was purified by antigen-affinity chromatography.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product may contain other non-IgG subtypes.
Additional Product Notes: The 50 µL size can be used for approximately 5 Western blots.
Product Citations: Calvo-López T, et al. 2023. Front Microbiol. 13:1063706. PubMed Liang W, et al. 2021. Oxid Med Cell Longev. 2021:5537737. PubMed Jin G, et al. 2020. Oxid Med Cell Longev. 7690845:2020. PubMed
RRID: AB_2272658 (BioLegend Cat. No. 627501)
Structure: Contains 8 cysteine residues involved in intra- and inter-chain disulfide bonds, platelet-derived growth factor (PDGF) family, produced as a homodimeric protein with an approximate molecular weight of 34-46 kD. Human VEGF is alternatively spliced to encod
Distribution: Widely expressed
Function: Mitogen for vascular endothelial cells, promotes angiogenesis and increases permeability of capillary blood vessels
Interaction: Heparin, many other proteins
Ligand/Receptor: VEGF-R1 (Flt-1) and VEGF-R2 (KDR)
Modification: Glycosylation
Biology Area: Cell Biology, Immunology
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: 1. Connolly DT, et al. 1989. J. Clin. Invest. 84:1470. 2. Drake CJ, et al. 1995. Proc. Natl. Acad. Sci. USA 92:7657. 3. Houck KA, et al. 1991. Mol. Endocrinol. 5:1806.
Gene ID: 7422
UniProt: View information about VEGF on UniProt.org
Clone: Poly6275
Regulatory Status: RUO
Other Names: Vascular endothelial growth factor, vasculotropin, vascular permeability factor
Isotype: Rabbit Polyclonal IgG