Biolegend, 627652, Purified anti-MAD2 Antibody, 200microl

MAD2 (mitotic arrest deficient 2) is a spindle checkpoint protein that regulates the progression of mitosis. First identified in yeast, there exist two orthologues in humans, MAD2L1 and MAD2L2, whose expression patterns are varied and often overlap. During mitosis MAD2 is recruited to unattached kinetochores and sequesters CDC20, preventing the activation of APC (anaphase promoting complex) and blocking progression from metaphase to anaphase. Thus, MAD2 acts as a check against aneuploidy by preventing the improper segregation of sister chromatids. This antibody is specific to the MAD2L1 homolog, not MAD2L2.
200microl
Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Rabbit
Immunogen: Recombinant full-length human MAD2L1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.05 - 0.25 µg/mL. For immunocytochemistry, a concentration range of 5.0 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody (clone Poly23A13) is not suitable for immunohistochemistry (IHC). This antibody (clone Poly23A13) is cross-reactive with mouse MAD2/MAD2L1 in western blot (WB). However, several prominent non-specific bands are observed around 42 kD when detecting mouse samples. By western blot (WB) this antibody recognizes only MAD2/MAD2L1, not its homolog MAD2L2. For immunocytochemistry (ICC), fixation with 4% PFA followed by permeabilization with 0.5% Triton-X is recommended. Fixation and permeabilization with 100% ice-cold methanol alone, or fixation with 4% PFA followed by permeabilization with 100% ice-cold methanol is not recommended due to mis-localization of signal.
RRID: AB_3097526 (BioLegend Cat. No. 627651) AB_3097526 (BioLegend Cat. No. 627652)
Structure: MAD2L1 is a 205 amino-acid protein with a predicted molecular weight of 23.5 kD.
Distribution: Primarily nuclear, kinetochores
Function: Mitosis, Spindle checkpoint
Interaction: Mad2l2, cdc20, APC
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Antigen References: Krishnan R, et al. 1998. Genomics. 49:475-8. Díaz-Martínez LA, et al. 2009. Cell Cycle. 8:2733-40. Hardwick KG. 2005. Curr Biol. 15:R122-4.
Gene ID: 4085
UniProt: View information about MAD2 on UniProt.org
Clone: Poly23A13
Regulatory Status: RUO
Other Names: Mitotic spindle assembly checkpoint protein MAD2A, mitotic arrest deficient 2-like protein 1, MAD2L1, HsMAD2
Isotype: Rabbit Polyclonal IgG