Biolegend, 627702, Purified anti-PLK-1 Antibody, 100μg

PLK-1 (polo-like kinase 1) is a member of te serine/threonine protein kinase family, cdc5/polo subfamily. Highly homologous to polo-like kinase (Drosophila), PLK-1 contains two polo box domains with a predicted molecular weight of 68 kD. This nuclear protein is highly expressed in placenta and colon and has been shown to regulate cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. PLK-1 may also be required for cell division; depletion of PLK-1 results in apoptosis. PLK-1 is upregulated by growth stimulating agents and is regulated by cell cycle position (highest in G2/M phase, declining to nearly undetectable levels after mitosis and throughout G1). PLK-1 is modified by phosphorylation (Thr210 is the major phosphorylation site in activated PLK-1 from mitotic cells) and has been shown to interact with nuclear distribution gene C. The 3F8 monoclonal antibody recognizes human and mouse PLK-1 and has been shown to be useful for Western blotting.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Amino Acid: 300-603 of human PLK-1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested KO/KD-WB - Verified IP, ICC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1.0 - 5.0 µg (1:100-1:500) antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Chilamakuri R, et al. 2022. Pharmaceuticals (Basel). 15:. PubMed Zhu K, et al. 2022. Cell Death Differ. 2070:29. PubMed Rajamanickam S, et al. 2016. Clin Cancer Res. 22: 3524 - 3536. PubMed Auler M, et al. 2020. J Invest Dermatol. . PubMed
RRID: AB_439756 (BioLegend Cat. No. 627701) AB_439757 (BioLegend Cat. No. 627702)
Structure: Serine/Threonine family of protein kinases, cdc5/polo subfamily. Highly homologous to polo-like kinase (Drosophila). Contains two polo box domains. Predicted molecular weight 68 kD
Distribution: Nuclear protein, highly expressed in placenta and colon
Function: Regulates cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. May be required for cell division. Depletion of PLK-1 results in apoptosis
Interaction: Interacts with nuclear distribution gene C
Modification: Phosphorylation
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: 1. Hamanaka R, et al. 1994. Cell Growth Differ. 5:249. 2. Lake RJ, et al. 1993. Mol. Cell. Biol. 13:7793. 3. Holtrich U, et al. 1994. P. Natl. Acad. Sci. USA 91:1736.
Regulation: Upregulated by growth stimulating agents. Regulated by cell cycle position (highest in G2/M phase and declines to nearly undetectable levels after mitosis and throughout G1)
Gene ID: 5347
UniProt: View information about PLK-1 on UniProt.org
Clone: 3F8
Regulatory Status: RUO
Other Names: Serine/Threonine protein kinase PLK, Polo-like kinase (PLK), Serine-threonine protein kinase 13
Isotype: Mouse IgG1, κ