Biolegend, 627902, Purified anti-Tubulin-α Antibody, 100μg

Tubulin, one of the major constituents of microtubules, is a dimeric protein consisting of an alpha and beta chain. Tubulin is a highly conserved protein that is ubiquitously expressed. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation. Tubulin interacts with a variety of proteins including RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, and microtubule associated protein 1A. The 10D8 antibody recognizes α-tubulin in all species and is useful for Western blotting, and immunofluorescence staining. The 10D8 antibody can also be used to monitor protein loading in Western blotting.
100μg
Verified Reactivity: Human, Mouse, Rat, All Species
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant full length human α-tubulin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified ICC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2 - 10 µg/mL is suggested. For immunofluorescent staining applications, a concentration range of 1 - 4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Chou HYE, et al. 2006. J. Biol. Chem. 281:15201.
Product Citations: Bian S, et al. 2021. Exp Ther Med. 22:667. PubMed Gärtner K, et al. 2018. Cancer Med. 11:39. PubMed Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed Kogut S, et al. 2022. Epigenomes. 6:. PubMed Rodems TS, et al. 2022. Commun Biol. 5:897. PubMed Yadav B, et al. 2021. PLoS Pathog. 17:e1009891. PubMed
RRID: AB_439760 (BioLegend Cat. No. 627901) AB_439761 (BioLegend Cat. No. 627902)
Structure: Tubulin family, tubulin is a dimeric protein consisting of an alpha and beta chain. Alpha tubulin has an approximate molecular weight of 50-55 kD.
Distribution: Ubiquitous
Function: Tubulin is one of the major constituents of microtubules. Binds GTP, alpha chain contains a non-exchangeable GTP binding site
Interaction: RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, Microtubule associated protein 1A
Modification: Phosphorylation, acetylation
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Microtubules
Antigen References: 1. Cowan NJ, et al. 1983. Mol. Cell. Biol. 3:1738. 2. Miller FD, et al. 1987. J. Cell Biol. 105:3065. 3. Wilde CD, et al. 1982. Proc. Natl. Acad. Sci. USA 79:96.
Gene ID: 84790
UniProt: View information about Tubulin-alpha on UniProt.org
Clone: 10D8
Regulatory Status: RUO
Other Names: α-tubulin, Tubulin, alpha-ubiquitous chain
Isotype: Mouse IgM, κ