Biolegend, 627905, Alexa Fluor® 488 anti-Tubulin-α Antibody, 25μg

Tubulin, one of the major constituents of microtubules, is a dimeric protein consisting of an alpha and beta chain. Tubulin is a highly conserved protein that is ubiquitously expressed. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation. Tubulin interacts with a variety of proteins including RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, and microtubule associated protein 1A. The 10D8 antibody recognizes α-tubulin in all species and is useful for Western blotting, and immunofluorescence staining. The 10D8 antibody can also be used to monitor protein loading in Western blotting.
25μg
Verified Reactivity: Human, Mouse, Rat, All Species
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant full length human α-tubulin
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistrys, a concentration range of 1.0 - 4.0 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Chou HYE, et al. 2006. J. Biol. Chem. 281:15201.
Product Citations: Ziegler CGK, et al. 2021. Cell. 184(18):4713-4733.e22. PubMed Khalin I, et al. 2020. ACS Nano. 14:9755. PubMed
RRID: AB_893643 (BioLegend Cat. No. 627905) AB_893642 (BioLegend Cat. No. 627906)
Structure: Tubulin family, tubulin is a dimeric protein consisting of an alpha and beta chain. Alpha tubulin has an approximate molecular weight of 50-55 kD.
Distribution: Ubiquitous
Function: Tubulin is one of the major constituents of microtubules. Binds GTP, alpha chain contains a non-exchangeable GTP binding site
Interaction: RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, Microtubule associated protein 1A
Modification: Phosphorylation, acetylation
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Microtubules
Antigen References: 1. Cowan NJ, et al. 1983. Mol. Cell. Biol. 3:1738. 2. Miller FD, et al. 1987. J. Cell Biol. 105:3065. 3. Wilde CD, et al. 1982. Proc. Natl. Acad. Sci. USA 79:96.
Gene ID: 84790
UniProt: View information about Tubulin-alpha on UniProt.org
Clone: 10D8
Regulatory Status: RUO
Other Names: α-tubulin, Tubulin, alpha-ubiquitous chain
Isotype: Mouse IgM, κ