Biolegend, 630252, Purified anti-mouse IL-7 Antibody, 100μg

Mouse IL-7 was initially described as a pre-B-cell grow factor expressed in bone marrow stromal cells. IL-7 is essential for normal murine B cell development, and plays a key role in regulating the homeostasis and function of the T-cells. The binding of IL-7 to its receptor induces dimerization of IL-7Ra and the common gamma chain (gc) which leads to activation of receptor associated tyrosine Janus kinases JAK1 (IL-7R) and JAK3 (γc). The activated JAK proteins in turn phosphorylate specific residues on the IL-7R creating docking sites for signaling molecules such as STAT5 and to a lesser extent STAT1 and STAT3. In adult mice, IL-7/IL-7R signaling up regulates expression of early B cell factor (EBF), and EBF in turn regulates expression of B cell-specific genes required for the transition from lymphoid progenitor to pro-B cells. Besides, IL-7 plays a role in the development of secondary lymphoid tissues. IL-7 is necessary to specify CD8 lineage differentiation during CD4/CD8 cell fate choice in the thymus by inducing expression of the transcription factor Runx3. IL-7 induces antiapoptotic factors Bcl2 and Bcl-xL and inhibiting pro-apoptotic factors such as Bad and Bax. In this fashion, IL-7 induces cell activation, survival, and proliferation of T lymphocytes. In addition, IL-7 controls T-cell size and metabolism through the activation of PI3 kinase-dependent pathways and regulation of glucose metabolism. IL-7 also controls T cell–dendritic cell interactions that are essential for both T-cell homeostasis and activation in vivo . CD4 T cell lymphopenia increases the expression of circulating IL-7, and TGFb induces IL-7 downregulation.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Recombinant IL-7 Protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: Direct ELISA - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.
Additional Product Notes: This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone A18052H) could function as a detection antibody when paired with purified anti-mouse IL-7 antibody (Cat. No. 630452) (clone A18052C) as the capture antibody and using Recombinant Mouse IL-7 protein (Cat Nos. 577802, 577804, 577806, and 577808) as the assay standard. For assay development flexibility purposes, this product is provided as a purified format.
RRID: AB_3097286 (BioLegend Cat. No. 630252)
Structure: Cytokine
Distribution: Thymic and bone morrow stromal cells, keratinocytes, dendritic cells, endothelial cells, intestinal epithelial, and epithelial goblet cells
Function: IL-7 is essential for B cell production
Interaction: Progenitor B and T cells, CD4-CD8- thymocytes, naïve and memory CD4 and CD8 T cells, and megakaryocytes
Ligand/Receptor: Heterodimer IL-7Rα (CD127); γ-subunit (CD132) which shares component with IL-2, IL-4, IL-13, IL-15, and IL-21 receptors
Cell Type: Embryonic Stem Cells
Biology Area: Cancer Biomarkers, Neuroinflammation, Neuroscience, Stem Cells
Antigen References: Yokota T, et al. 1986. P Natl Acad Sci USA. 83:5894. Meier D, et al. 2007. Immunity 26:643-54. Parish JK, et al. 2009. J Immunol. 182:4255-66. Saini M, et al. 2009. Blood. 113:5793-800. Park JH, et al. 2010. Nat Immunol. 11:257-64. Li WQ, et al. 2010. Mol Cell Biol. 30:590-600.
Gene ID: 16196
UniProt: View information about IL-7 on UniProt.org
Clone: A18052H
Regulatory Status: RUO
Other Names: Interleukin-7, Lymphopoietin-1 (LP-1), Pre-B cell growth factor, Thymocyte growh factor
Isotype: Rat IgG2a, κ