Biolegend, 630302, Purified anti-AND-1 (WDHD1) Antibody, 100μg

The 20G10 monoclonal antibody recognizes both human and mouse AND-1 (WDHD1) also known as WD repeat and HMG box DNA binding protein 1, acidic nucleoplasmic DNA-binding protein 1. AND-1 (WDHD1) is a nuclear and nucleolar protein that contains multiple WD40 repeats, and one HMG region. AND-1 (WDHD1) has a predicted molecular weight of 126 kD and can form homodimers. WDHD1 is expressed in the brain, epidermis, liver, and stomach. During mitosis, AND-1 (WDHD1) is transiently expressed in nucleoplasm. AND-1 (WDHD1) can bind to DNA with high affinity and may be involved in protein-protein interactions. The role of AND-1 (WDHD1) is thought to be chromatin assembly, transcription and DNA replication. AND-1 (WDHD1) has been reported to be phosphorylated on S1041. The 20G10 antibody has been shown to be useful for Western blotting.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant (partial), C-terminal
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration of 15 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Prakash Yadav R, et al. 2023. Reproduction. 165:49. PubMed Kim B, et al. 2020. Molecular Cell. 78(4):624-640. PubMed Jeong DS, et al. 2022. Cell J. 24:51. PubMed
RRID: AB_2215084 (BioLegend Cat. No. 630301) AB_2215083 (BioLegend Cat. No. 630302)
Structure: Contains multiple WD40 repeats, one HMG region. Predicted molecular weight 126 kD. Forms homodimers.
Distribution: Found in the nucleus and nucleolus. Expressed in brain, epidermis, liver, and stomach. During mitosis transiently expressed in nucleoplasm.
Function: Binds DNA with high affinity; may be involved in protein-protein interactions. Thought to function in chromatin assembly, transcription and DNA replication.
Modification: Phosphorylation (S1041)
Biology Area: Cell Biology
Molecular Family: Nuclear Markers
Antigen References: 1. Kohler A, et al. 1997. J. Cell Sci. 110:1051.
Gene ID: 11169
UniProt: View information about AND-1 on UniProt.org
Clone: 20G10
Regulatory Status: RUO
Other Names: WD repeat and HMG box DNA binding protein 1, acidic nucleoplasmic DNA-binding protein 1
Isotype: Mouse IgG2a, κ