Biolegend, 631252, Purified anti-c-Fos Phospho (Ser32) Antibody, 100μg

The proto-oncogene c-Fos is the human homolog of the retroviral v-Fos oncogene, and is a member of the Fos family of transcription factors. c-Fos forms heterodimers with proteins of the Jun family of transcription factors to form the Activator Protein-1 complex (AP-1). This complex is involved in critical cellular processes such as inflammation, cell proliferation, differentiation, survival, hypoxia, bone development and angiogenesis which suggests c-Fos is an important therapeutic target for cancer, vascular remodeling, acute inflammation and autoimmune diseases.  In addition, increases in c-Fos expression and activation are associated with neuronal activity induced by psychoactive drugs. Expression of c-Fos is rapid and transitory, and phosphorylation at residues Ser32 and Thr232 by Erk5 increases protein stability and nuclear localization.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide from human c-Fos phosphorylated at Ser32.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, ICFC, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.012 - 0.1 µg/mL. For immunocytochemistry, a concentration range of 2.5 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For ICFC use we recommend cell fixation/permeabilization with True-Phos™ Perm buffer.
RRID: AB_3097545 (BioLegend Cat. No. 631251) AB_3097545 (BioLegend Cat. No. 631252)
Structure: c-Fos is a 380 amino acid length protein. It has a leucine zipper domain that allows it to form heterodimers with members of the Jun family proteins.
Distribution: Non-phosphorylated forms can be found in the endoplasmic reticulum. However, after activation/phosphorylation the protein is translocated to the nucleus.
Function: c-Fos is a subunit of the AP-1 transcription factor. The half-life of the protein is short; however, phosphorylation induces protein stability and translocation to the nucleus.
Interaction: c-Fos interacts with members of the Jun family proteins to form the AP-1 transcription factor.
Ligand/Receptor: As a part of the transcription factor AP-1, it binds to specific DNA sequences to regulate gene transcription
Biology Area: Cell Biology, Cell Proliferation and Viability, Immuno-Oncology, Immunology, Innate Immunity, Neuroscience, Transcription Factors
Molecular Family: Innate Immune Signaling, Phospho-Proteins, Tumor Suppressors
Antigen References: Sasaki T, et al. 2006. Mol Cell. 24:63-75. Huang K, et al. 2014. Cell Signal. 26:629-38. Qu X, et al. 2019. Cancer Sci. 110:3183-3196. Guo JC, et al. 2015. PLoS One. 10:e0120332. Rodriguez-Berdini L, et al. 2020. J Biol Chem. 295:8808-8818.
Gene ID: 2353
UniProt: View information about c-Fos on UniProt.org
Clone: A22009C
Regulatory Status: RUO
Other Names: G0S7, p55, cFos, c-Fos, Proto-oncogene, Transcription Factor subunit, AP-1
Isotype: Mouse IgG2b, κ