Biolegend, 631254, PE anti-c-Fos Phospho (Ser32) Antibody, 100tests

The proto-oncogene c-Fos is the human homolog of the retroviral v-Fos oncogene, and is a member of the Fos family of transcription factors. c-Fos forms heterodimers with proteins of the Jun family of transcription factors to form the Activator Protein-1 complex (AP-1). This complex is involved in critical cellular processes such as inflammation, cell proliferation, differentiation, survival, hypoxia, bone development and angiogenesis which suggests c-Fos is an important therapeutic target for cancer, vascular remodeling, acute inflammation and autoimmune diseases.  In addition, increases in c-Fos expression and activation are associated with neuronal activity induced by psychoactive drugs. Expression of c-Fos is rapid and transitory, and phosphorylation at residues Ser32 and Thr232 by Erk5 increases protein stability and nuclear localization.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide from human c-Fos phosphorylated at Ser32.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: For ICFC use we recommend cell fixation/permeabilization with True-Phos™ Perm buffer.
RRID: AB_3106233 (BioLegend Cat. No. 631253) AB_3106233 (BioLegend Cat. No. 631254)
Structure: c-Fos is a 380 amino acid length protein. It has a leucine zipper domain that allows it to form heterodimers with members of the Jun family proteins.
Distribution: Non-phosphorylated forms can be found in the endoplasmic reticulum. However, after activation/phosphorylation the protein is translocated to the nucleus.
Function: c-Fos is a subunit of the AP-1 transcription factor. The half-life of the protein is short; however, phosphorylation induces protein stability and translocation to the nucleus.
Interaction: c-Fos interacts with members of the Jun family proteins to form the AP-1 transcription factor.
Ligand/Receptor: As a part of the transcription factor AP-1, it binds to specific DNA sequences to regulate gene transcription
Biology Area: Cell Biology, Cell Proliferation and Viability, Immuno-Oncology, Immunology, Innate Immunity, Neuroscience, Transcription Factors
Molecular Family: Innate Immune Signaling, Phospho-Proteins, Tumor Suppressors
Antigen References: Sasaki T, et al. 2006. Mol Cell. 24:63-75. Huang K, et al. 2014. Cell Signal. 26:629-38. Qu X, et al. 2019. Cancer Sci. 110:3183-3196. Guo JC, et al. 2015. PLoS One. 10:e0120332. Rodriguez-Berdini L, et al. 2020. J Biol Chem. 295:8808-8818.
Gene ID: 2353
UniProt: View information about c-Fos on UniProt.org
Clone: A22009C
Regulatory Status: RUO
Other Names: G0S7, p55, cFos, c-Fos, Proto-oncogene, Transcription Factor subunit, AP-1
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.