Biolegend, 631352, Purified anti-CD102 (ICAM-2) Antibody, 100μg

CD102 (ICAM-2) is a 55 kD type I transmembrane glycoprotein which belongs to the immunoglobulin superfamily. It is also known as Intercellular Adhesion Molecule-2, or ICAM-2. CD102 is constitutively expressed on vascular endothelial cells, some lymphocytes, monocytes, and platelets. CD102 is thought to be involved in lymphocyte recirculation, and in co-stimulation of the immune response. CD102 and CD54 in endothelial cells, such as in blood-brain barrier, are essential for assisting T cell polarization and crawling and diapedesis. CD102 is a counter receptor of LFA-1 (CD11a/CD18), DC-SIGN and MAC-1.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CD102 (ICAM-2) recombinant protein fragment
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested ICC, WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1 - 10 μg/mL is recommended. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For western blotting (WB) it is recommended to heat the cell lysate at 37 °C for 30 min rather than boiling it. For immunocytochemistry (ICC), either Fixation Buffer (Cat. No. 420801) or 4% paraformaldehyde followed by methanol permeabilization is recommended. The reactivity of this antibody (clone W22039C) in cell surface flow cytometry is able to be blocked by another BioLegend antibody (clone CBR-IC2/2) (Cat. No. 328502).
RRID: AB_3097547 (BioLegend Cat. No. 631351) AB_3097547 (BioLegend Cat. No. 631352)
Structure: 55 kD type I transmembrane glycoprotein, Ig superfamily
Distribution: Vascular endothelial cells, lymphocytes, monocytes, platelets
Function: Adhesion, costimulation
Ligand/Receptor: LFA-1, DC-SIGN, MAC-1
Cell Type: Endothelial cells, Lymphocytes, Monocytes, Platelets
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Weber KSC, et al. 2004. Inflammation. 28:177-88. Barclay AN, et al. 1997. The Leukoycte Antigen Factsbook. Casasnovas JM, et al. 1999. Proc Natl Acad Sci USA. 96:3017-22. Diamonds MS, et al. 1990. J Cell Biol. 111:3129-39. Marchetti L & Engelhardt B. 2020. Vasc Biol. 2:H1-H18.
Gene ID: 3384
UniProt: View information about CD102 on UniProt.org
Clone: W22039C
Regulatory Status: RUO
Other Names: Intercellular Adhesion Molecule-2, ICAM-2
Isotype: Mouse IgG2b, κ