Biolegend, 631452, Purified anti-human PLAUR (uPAR) Antibody, 100μg

PLAUR (plasminogen activator urokinase receptor), also known as urokinase plasminogen activator-receptor (uPAR), is part of the plasminogen activation system, which is involved in tissue reorganization, cell migration, cell cycle regulation, and cell adhesion. PLAUR was originally identified as a saturable binding site for urokinase on the cell surface, and thus, restricting plasminogen activation to the immediate vicinity of the cell membrane. Due to lack of transmembrane or cytoplasmic domains, PLAUR may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) moiety following cleavage of the nascent polypeptide near its C-terminus. A soluble form of PLAUR has been identified. The membrane-bound PLAUR is expressed on granulocytes, monocytes/macrophages, dendritic cells, fibroblasts, endothelial cells, and keratinocytes. This molecule plays a role in cell adhesion and chemotaxis through binding to β1, β2, and β3 integrins. Recently, it was found that soluble PLAUR is increased in plasma of patients with active lupus nephritis, suggesting an association with pathological features of the disease. In addition, soluble PLAUR has been linked as a causative factor and also a biomarker of several diseases including tumor metastasis, focal and segmental glomerulosclerosis, hepatocellular carcinoma, and coronary artery disease.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Recombinant PLAUR (uPAR) Protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: Direct ELISA - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.
Additional Product Notes: This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone A20095T) could function as a detection antibody when paired with purified anti-human PLAUR (uPAR) (Cat No. 632152) (clone A20095I) as the capture antibody and using Recombinant Human PLAUR (uPAR) (carrier-free) protein (Cat. Nos. 559702 and 559706) as the assay standard. For assay development flexibility purposes, this product is provided as a purified format.
RRID: AB_3683477 (BioLegend Cat. No. 631452)
Structure: Monomer
Distribution: Granulocytes, monocytes/macrophages, dendritic cells, fibroblasts, endothelial cells, keratinocytes, and activated T cells
Function: Convert plasminogen to plasmin, cell adhesion, and chemotaxis
Ligand/Receptor: uPA; LRP1 (CD91)
Cell Type: Embryonic Stem Cells
Biology Area: Angiogenesis, Cell Biology, Stem Cells
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: Llinas P, et al. 2005. EMBO J. 24:1655-63. Beaufort N, et al. 2004. J Immunol. 172:540-9. Tarui T, et al. 2001. J Biol Chem. 276:3983-90. Qin DD, et al. 2014. Lupus. 24:546-57. Thuno M, et al. 2009. Dis Markers. 27:157-72. Alfano D, et al. 2009. Front Biosci. 14:3782-94. Spinale JM, et al. 2014. Kidney Int. 87:564-74. Chounta A, et al. 2014. Liver Int. 35:601-7. Harskamp RE and Roe MT. 2014. J Am Heart Assoc. 3:e001431.
Gene ID: 5329
UniProt: View information about PLAUR on UniProt.org
Clone: A20095T
Regulatory Status: RUO
Other Names: CD87, MC3, Plasminogen activator urokinase receptor, uPAR, URKR
Isotype: Mouse IgG2a, κ