Biolegend, 631552, Purified anti-mouse IL-15 Antibody, 100μg

IL-15 was discovered in the supernatant of Simian kidney epithelial cell line CV-1/EBNA, as a soluble factor capable of supporting proliferation of the IL-2-dependent cell line CTLL-2. IL-15 is a regulatory cytokine, and it is produced by dendritic cells, epithelial cells, fibroblasts, and monocytes. IL-15 plays an important role in immune response and shares many functions with IL-2. For example, it stimulates the proliferation of activated T cells, NK cells, and B cells, and induces immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand. In addition, IL-15 promotes the development of dendritic cells and induces the production of proinflammatory cytokines from macrophages. IL-15 acts as a bridge between innate and adaptive immunity because of its diverse roles in the immune system. IL-15 binds to heterotrimeric receptors composed of IL-15Rα, IL-15Rβ, and IL-15Rγc. IL-15 shares the receptor chains β and γc with IL-2. IL-15 is normally not secreted in soluble form but is instead held on the cell surface bound to a unique receptor, IL-15Rα, especially on dendritic cells. Cell-bound IL-15 is then presented in trans form to T cells and NK cells and is recognized by the γc receptor on these cells; such recognition maintains cell survival and intermittent proliferation.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Recombinant IL-15 Protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: Direct ELISA - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.
RRID: AB_3675171 (BioLegend Cat. No. 631552)
Structure: Cytokine
Distribution: IL-15 is expressed by DC, epithelial cells, fibroblasts, and monocytes.
Function: IL-15 stimulates the proliferation of activated T cells, NK cells, and B cells, and inducing immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand. In addition, IL-15 promotes the development of dendritic cells, and induces the production of proinflammatory cytokines from macrophages.
Ligand/Receptor: IL-15Rα, IL-15Rβ and IL-15γc
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Cytokines/Chemokines
Antigen References: Grabstein K, et al. 1994. Science. 264:965-8. Ma A, et al. 2006. Annu Rev Immunol. 24:657-79. Meresse B, et al. 2004. Immunity. 21:357-66. Armitage RJ, et al. 1995. J Immunol. 154:483-90. Pulendran B, et al. 2004. Eur J Immunol. 34:66-73. Bouchard A, et al. 2004. J Leukoc Biol. 75:893-900. Ratthe C, et al. 2004. J Leukoc Biol. 75:893-900. Feng T, et al. 2008. Cell Immunol. 5:189. Rubinstein MP, et al. 2006. P Natl Acad Sci USA. 103:9166.
Gene ID: 16168
UniProt: View information about IL-15 on UniProt.org
Clone: W19387G
Regulatory Status: RUO
Other Names: IL-15
Isotype: Rat IgG2a, κ