Biolegend, 631752, Purified anti-Profilin-1 Antibody, 100μg

Profilin-1 is an actin-binding protein responsible for the regulation of actin dynamics. Profilin-1 assists in the exchange of ADP for ATP on G-actin, facilitating the polymerization of actin monomers into filaments by promoting assembly at the growing end. Thus, profilin-1 is implicated in processes like cell motility, cytokinesis, and endocytosis, highlighting its significance in maintaining cellular structure and function. Profilin-1 also interacts with numerous proteins involved in signaling pathways, influencing cellular responses to intracellular and extracellular cues. Emerging evidence also shows that profilin-1 can bind to microtubules, facilitating interactions between the two major components of the cytoskeleton. Mutations in profilin-1 have been associated with amyotrophic lateral sclerosis (ALS) and is suspected to cause insoluble aggregates due to disruption in axonal outgrowth, cell integrity and protein distribution.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant fragment of the N terminus of human Profilin 1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody (clone A18224E) is not suitable for immunocytochemistry (ICC). For immunohistochemistry (IHC), we recommend antigen retrieval with Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0). Antigen retrieval with Sodium Citrate is not recommended as it results in mis-localized signal.
RRID: AB_3097566 (BioLegend Cat. No. 631751) AB_3097566 (BioLegend Cat. No. 631752)
Structure: Profilin-1 is an 140 amino-acid protein with a predicted molecular weight of 15 kD.
Distribution: Ubiquitous, cytoskeleton/cytoplasm
Function: Actin dynamics, cell shape and motility
Interaction: Actin, Ena/VASP proteins, phosphoinositides, ROCK, RAP1, CDC42
Ligand/Receptor: Profilin-1 binds actin monomers
Biology Area: Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Structure
Molecular Family: Microfilaments
Antigen References: Murk K, et al. 2021. Front Cell Dev Biol. 9:681122. Karlsson R, et al. 2021. J Cell Physiol. 236:7256-7265. Davey RJ, et al. 2020. Biophys Rev. 12:827-849.
Gene ID: 5216
UniProt: View information about Profilin-1 on UniProt.org
Clone: A18224E
Regulatory Status: RUO
Other Names: Profilin 1, PFN1, Profilin1, Epididymis tissue protein Li 184a
Isotype: Mouse IgG2b, κ