Biolegend, 632052, Purified anti-IRAK1 Antibody, 100μg

The interleukin-1 receptor-associated kinase (IRAK) family of proteins are made up of serine/threonine-specific kinases which play a critical role in the response to pathogens through the induction of acute inflammation and subsequent adaptive immune responses. The mammalian IRAK molecular family consists of four members (IRAK1, IRAK2, IRAK3/IRAK-M, and IRAK4). Two are active kinases, IRAK1 and IRAK4, and two are inactive kinases, IRAK2 and IRAKM. However, all are involved in the regulation of the nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways. The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1R1, IL-1RAcP, MyD88, and IRAKs. Currently, there are three differentially spliced variants of IRAK1: IRAK1, IRAK1b, and IRAK1c. Auto-phosphorylation plays a role in IRAK-1 activation and mediates proteasome-mediated degradation leading to IRAK1 protein loss. Meanwhile, IRAK1b lacks kinase activity and is resistant to proteasome-mediated degradation. Additionally, IRAK1c has a truncated sequence and is therefore mutated at the C-terminus of its kinase domain and acts as a negative regulator of the TLR and IL-1R signaling pathways.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human IRAK1 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1 - 5 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For immunocytochemistry (ICC), we recommend using 4% PFA fixation followed by permeabilization with 0.5% Triton X-100 or ice cold-methanol, or ice-cold methanol fixation. This product is not recommended for use in immunohistochemistry (IHC).
Additional Product Notes: This antibody has been tested in knockout/knockdown models for Western Blotting (WB).
RRID: AB_3097576 (BioLegend Cat. No. 632051) AB_3097576 (BioLegend Cat. No. 632052)
Structure: IRAK1 protein is a 712 amino acid protein with a predicted molecular weight of 77 kD
Distribution: Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
Function: Serine/threonine protein kinase
Interaction: TRAF6, PELI1, IRAK4, MYD88, TOLLIP, IL1RL1, IKBKG/NEMO, IRAK4, PELI3, INAVA and NFATC4
Cell Type: Macrophages, Monocytes
Biology Area: Cell Biology, Immunology, Innate Immunity
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Dinarello CA. 1996. Blood. 87:2095-147. Takaesu G, et al. 2001. Mol Cell Biol. 21: 2475-84. Janssens S & Beyaert R. 2003. Mol Cell. 11:293-302. Jain A, et al. 2014. Front Immunol. 5:553. Gottipati S, et al. 2008. Cell Signal. 20:269-76.
Gene ID: 3654
UniProt: View information about IRAK1 on UniProt.org
Clone: W22166E
Regulatory Status: RUO
Other Names: AA48924; Il1rak; Interleukin 1 receptor associated kinase 1; IRAK; IRAK-1
Isotype: Rat IgG2a, κ