Biolegend, 632351, Purified anti-CD38 Antibody, 25μg

CD38 is a widely-expressed, multifunctional protein whose role is best studied in the immune system, where it is expressed at varying levels in multiple immune cell types including lymphocytes, monocytes/macrophages, dendritic cells and NK cells. CD38 functions in cell signaling via protein-protein interactions with cell membrane proteins in other immune cell types. For example, CD38 interacts with the T cell receptor complex in T cells and the B cell co-receptor complex in B cells. CD38 also binds to CD31/PECAM in blood vessels to mediate lymphocyte adhesion. Additionally, CD38 has intrinsic enzymatic activity, catalyzing the synthesis of ADP ribose, cyclic ADP ribose, or nicotinamide from NAD. Due to the influence the synthesized molecules have on calcium dynamics, CD38 plays a role in diverse physiological processes, including regulating insulin secretion and modulation of neurotransmitter release.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human CD38
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody (clone W22190F) is not suitable for flow cytometry (FC) or intracellular flow cytometry (ICFC). For immunohistochemistry (IHC), we recommend antigen retrieval with either Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0) or sodium citrate (Cat. No. 928502). For immunocytochemistry (ICC), we recommend fixation/permeabilization with either 100% ice-cold methanol, or fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with 0.5% Triton-X or 100% ice-cold methanol.
RRID: AB_3097610 (BioLegend Cat. No. 632351) AB_3097610 (BioLegend Cat. No. 632352)
Structure: CD38 is a 300 amino acid protein with a predicted molecular weight of 34.3 kD
Distribution: Cell surface/plasma membrane
Function: Receptor/co-receptor, enzyme
Interaction: CD16, T cell receptor, B cell receptor, class II MHC, CD31
Cell Type: B cells, Dendritic cells, NK cells, Plasma cells, T cells
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Glaria E, et al. 2020. Cells. 9:228. Piedra-Quintero ZL, et al. 2020. Front Immunol. 11:597959. Li Y, et al. 2020. Future Oncol. 16:2853-2861.
Gene ID: 952
UniProt: View information about CD38 on UniProt.org
Clone: W22190F
Regulatory Status: RUO
Other Names: cluster of differentiation 38 (CD38), ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 (ADPRC1/cADPR hydrolase 1), 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase
Isotype: Rat IgG2a, κ