Biolegend, 632653, Ultra-LEAF™ Purified anti-human CD134 (OX40) Antibody, 100μg

OX40 (ACT35, CD134, TNFRSF4) is an approximately 48 kD transmembrane protein belonging to the TNF receptor superfamily. It is a 249 amino acid glycoprotein with a 41 amino acid cytoplasmic tail, 20 amino acid transmembrane domain, and a 185 amino acid extracellular domain. OX40 is a costimulatory molecule implicated in long-term T cell immunity. It is a receptor for TNFSF4, OX40L, GP34, and also acts as a receptor for human herpesvirus 6B/HHV-6B. OX40 is primarily expressed on activated T cells, including CD4 and CD8 T cells, Th1, Th2, and Th17 cells, and regulatory T cells. The main source of OX40L is antigen presenting cells. OX40L can also be induced on activated B cells, mature dendritic cells, Langerhans cells, and macrophages. OX40 and OX40L interactions regulate the division and survival of T cells. It inhibits the conversion of effector T cells into regulatory T cells (Tregs) and promote the recall response in memory T cells. OX40 and OX40L interactions are also involved in the development of multiple diseases including allergic airway inflammation, graft-versus-host disease and autoimmune disease.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human OX40/TNFRSF4-Fc
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: < 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Block - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by blocking the binding of 0.05 µg/mL recombinant human OX40/TNFRSF4-Fc chimera (Cat. No. 780804) to immobilized 1 µg/mL recombinant human OX40L (Cat. No. 555706). ND50 = 4 - 24 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3097584 (BioLegend Cat. No. 632653) AB_3097584 (BioLegend Cat. No. 632654)
Structure: Disulfide linked homodimer
Distribution: Primarily expressed on the activated T cells, including CD4 and CD8 T cells, Th1, Th2, and Th17 cells, and regulatory T cells
Function: Is a costimulatory molecule implicated in long-term T-cell immunity
Interaction: Antigen presenting cells
Ligand/Receptor: TNFSF4, OX40L, GP34, human herpesvirus 6B/HHV-6B
Cell Type: T cells, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Costimulatory Molecules, Immunology
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: Baum PR, et al. 1994. Circ Shock. 44:30-4. Bansal-Pakala P, et al. 2001. Nat Med. 7:907-12. Humphreys IR, et al. 2003. J Exp Med. 198:1237-42. Shimojima M, et al. 2004. Science. 303:1192-5. Tang H, et al. 2013. Proc Natl Acad Sci USA. 110:9096-9. Jacquemin C, et al. 2015. Immunity. 42:1159-70.
Gene ID: 7293
UniProt: View information about CD134 on UniProt.org
Clone: W22033K
Regulatory Status: RUO
Other Names: Tumor necrosis factor receptor superfamily member 4, TNFRSF4, ACT35, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, CD134
Isotype: Rat IgG2b, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.