Biolegend, 633001, Purified anti-Cbl Antibody, 125microl

The proto-oncogene c-Cbl was initially identified as the cellular homologue of v-Cbl oncogene that induces pre-B cell lymphomas and myeloid leukemias in mice. The Cbl family comprises three mammalian proteins, c-Cbl, Cbl-b, and Cbl-3; among these, c-Cbl and Cbl-b are expressed in hemopoietic cells, whereas Cbl-3 is expressed only in epithelial tissues. Cbl proteins can function as E3 ubiquitin (Ub) ligases, and in more recent studies Cbl has been implicated in the negative regulation of various RTKs.
125microl
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Polyclonal
Host Species: Rabbit
Immunogen: peptide mapping to the carboxy terminal domain of human Cbl
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% gelatin.
Preparation: The antibody was purified by affinity chromatography.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting suggested working dilution(s): Use 5 µl per 5 ml antibody (1:1000) dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This polyclonal was generated using an immunogen that corresponds to the C-terminus of c-Cbl. Due to poor sequence homology between the C-termini of c-Cbl and other Cbl family members, we do not predict this antibody will cross-react with other Cbl proteins. At concentrations greater than a 1:1000 dilution, this antibody may produce minor non-specific bands in certain lysates.
Additional Product Notes: The 125 µL size can be used for approximately 25 Western blots.
RRID: AB_2070439 (BioLegend Cat. No. 633001)
Structure: c-Cbl is a 906 amino acid protein with a predicted molecular weight of 99.6 kDa. The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. C-terminal contains proline-rich domain with potential tyrosine phosphorylation sites.
Distribution: Ubiquitous cytoplasmic protein
Interaction: NCK, CD2AP, SLA, SLA2, EGFR, SYK, LAT2 and ZAP70
Bioactivity: E3 ubiquitin (Ub) ligases, and negative regulators of PTKs
Biology Area: Cell Biology, Ubiquitin/Protein Degradation
Antigen References: 1. Liu YC, et al. 1998. Cell Signal 10:377.
Gene ID: 867
UniProt: View information about Cbl on UniProt.org
Clone: Poly6330
Regulatory Status: RUO
Other Names: E3 ubiquitin-protein ligase Cbl, Proto-oncogene c-Cbl
Isotype: Rabbit Polyclonal IgG