Biolegend, 633151, Purified anti-Dynamin-2 Antibody, 25μg

Dynamin-2 (DNM2) belongs to the dynamin family. It consists of GTPase domain (G-domain) at the N-terminus, middle domain, the lipophilic pleckstrin homology [PH] domain, GTPase effector domain (GED), and proline rich domain (PRD) at the C-terminus. The PH domain interacts with membrane phosphoinositides, while PRD domain interacts with SH3 domain-containing proteins. Dynamin-2 is a large mechanochemical GTPase involved in various cellular processes such as endocytosis, apoptosis, cytokinesis, phagocytosis, neuronal morphology, axonal growth, cell migration, cell membrane trafficking and cytoskeletal regulation. The studies indicate that mutations in Dynamin-2 cause centronuclear myopathy (CNM), Charcot-Marie-Tooth disease dominant intermediate type B (CMTDIB), hereditary spastic paraplegia and lethal congenital contractures syndrome type 5 (LCCS5). Additionally, numerous studies have implicated Dynamin-2 overexpression in several types of cancer and metastasis.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human Dynamin-2 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IHC-P, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunohistochemistry, a concentration range of 0.2 - 5.0 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.0625 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The clone does not cross-react with mouse in western blotting (WB). For immunocytochemistry (ICC), we recommend to use 4% PFA fixation followed by permeabilization with ice cold-methanol, or ice-cold methanol fixation. For immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), antigen retrieval with Citrate Buffer (Cat. No. 420901) or Tris-EDTA pH 9.0 is recommended. For intracellular staining for flow cytometry (ICFC), the recommended dosage is 0.0625 μg per test using intracellular staining buffer (Cat. No. 421002), Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803), True-Phos™ Perm Buffer (Cat. No. 425401) and True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401).
Additional Product Notes: This antibody has been tested in knockout/knockdown models for WB, ICC and ICFC.
RRID: AB_3097580 (BioLegend Cat. No. 633151) AB_3097580 (BioLegend Cat. No. 633152)
Structure: Dynamin-2 protein is a 870 amino acid protein with a predicted molecular weight of 98 kD.
Distribution: Ubiquitously expressed
Function: Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones. Plays an important role in vesicular trafficking processes, in particular endocytosis. Dynamin-2 is also involved in cytokinesis.
Interaction: MYOF, CTTN, ACTN1, SHANK1, SHANK2, SH3BP4 and NOSTRIN, SNX9, SNX18, SNX33, MYO1E, PSTPIP1, CTNND2, PIK3C3, BIN1
Cell Type: Neurons
Biology Area: Cell Biology, Neurodegeneration, Neuroscience
Molecular Family: Cytoskeletal Proteins
Antigen References: Cao H, et al. 1998. Mol Biol Cell. 9:2595-609. Gu C, et al. 2010. EMBO J. 29:3593-606. Bitoun M, et al. 2006. Med Sci. 22:101-2. Sidiropoulos P.N.M, et al. 2012. Brain. 135:1395-411. Sambuughin N, et al. 2015. BMC Neurol. 15:223. Koutsopoulos OS, et al. 2012. European Journal of Human Genetics. 21:637. Trochet D, et al. 2021. J Exp Clin Cancer Res. 40:238.
Gene ID: 1785
UniProt: View information about Dynamin-2 on UniProt.org
Clone: W22024F
Regulatory Status: RUO
Other Names: DYN2, DI-CMTB, CMTDIB, CMTDI1, DYNII, CMT2M, Dynamin II, LCCS5, Dynamin 2, DNM2
Isotype: Rat IgG2a, κ