Biolegend, 633252, Purified anti-DBN1 Antibody, 100μg

Drebrin (DBN1) is an actin-binding protein that was identified as a developmentally regulated protein expressed in the brain. Drebrin interacts with microtubules and is localized to focal adhesions, adherens junctions, gap junctions, immunological synapses, and neuronal synapses to modulate cellular processes such as cell migration, cell process formation, cancer metastasis, and spermatogenesis. There are two isoforms of mammals as a result of alternative splicing: Drebrin A (adult) and Drebrin E (embryonic). The expression of Drebrin A is highly specific to neurons and regulates the reorganization of actin filaments and thus contributes to memory, whereas Drebrin E is expressed in various tissues. Studies have shown that a decrease in the amount of Drebrin protein in the brain is thought to be a factor contributing to the pathogenesis of dementia in Alzheimer's disease (AD), Down syndrome (DS), and bipolar disorder (BD).
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human DBN1 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The clone does not cross-react with mouse in western blotting (WB). For immunocytochemistry (ICC), we recommend to use 4% PFA fixation followed by permeabilization with 0.5% Triton X-100 or ice cold-methanol, or ice-cold methanol fixation. For intracellular flow cytometry (ICFC), the recommended dosage is 0.06 μg per test using Fixation Buffer (Cat. No. 420801) followed by True-Phos™ Perm Buffer (Cat No. 425401), or Intracellular Staining Permeabilization Wash Buffer (Cat No. 421002), or using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). This product is not recommended for use in immunohistochemistry (IHC).
Additional Product Notes: This antibody has been tested in knockout/knockdown models for ICC.
RRID: AB_3097581 (BioLegend Cat. No. 633251) AB_3097581 (BioLegend Cat. No. 633252)
Structure: DBN1 protein is a 649 amino acid protein with a predicted molecular weight of 71 kD.
Distribution: Expressed in the brain, the heart, lymphocytes, placenta, lung, skeletal muscle, kidney, pancreas, skin fibroblasts, gingival fibroblasts, and bone-derived cells
Function: Cytoplasmic actin-binding protein, regulate dendritic spine morphogenesis and organization
Interaction: RUFY3, CXCR4, ZMYND8
Cell Type: Endothelial cells, Lymphocytes, Monocytes, Platelets
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Kojima N, et al. 1993. Brain Res Mol Brain Res. 19:101-14. Ishikawa R, et al.1994. J Biol Chem.269:29928-33. Butkevich E, et al.2004. Curr Biol. 14:650-8. Shirao T, et al. 2017. J Neurochem. 141:819-834. Peitsch WK, et al. 1999. Eur J Cell Biol. 78:767-78. Li MW, et al. 2011. Spermatogenesis. 1:123-136. Geraldo S, et al. 2008. Nat Cell Biol. 10:1181-9. Grintsevich EE, et al. 2017. Adv Exp Med Biol. 1006:61-82. Stiber J.A, et al. 2016. Arterioscler Thromb Vasc Biol.36:984-93. Shim K.S, et al. 2002. Neurosci Lett. 324:209-12. Kim H.W, et al. 2010. Neurobiol Dis. 37:596-603.
Gene ID: 1627
UniProt: View information about DBN1 on UniProt.org
Clone: W21139F
Regulatory Status: RUO
Other Names: DBN-1, DBN 1, D0S117E; Developmentally-regulated brain protein; Drebrin 1; Drebrin; Drebrin E; Drebrin E2; DKFZp434D064
Isotype: Rat IgG2b, κ