Biolegend, 633509, Alexa Fluor® 647 anti-Bcl-2 Antibody, 25μg

Bcl-2 (B-cell leukemia 2) is an apoptotic protein and a member of the Bcl-2 family containing BH1-4 domains. Two reported isoforms exist α=25 kD; β=22 kD. The Bcl-2 protein forms homo- or hetero-dimers with other Bcl-2 family members. Bcl-2 is distributed in the outer mitochondrial membrane, the nuclear envelope, and the endoplasmic reticulum. This protein blocks apoptotic death by controlling mitochondrial membrane permeability. Cleavage of Bcl-2 can convert to pro-apoptotic (by cleavage of BH4 domain). Bcl-2 has been reported to regulate cell cycle progression via ROS. This protein is modified by ASK1/JNK1, PKC, ERKs, and stress-activated kinase phosphorylation and can be ubiquitinated. Bcl-2 has been shown to interact with Apaf-1, Raf-1, TP53BP2, caspase-3, and form heterodimers with Bax, Bad, Bak, Bcl-x L , and Bag-1. Clone BCL/10C4 has been shown to be useful for Western blotting, immunoprecipitation, and immunofluorescence of the mouse and rat Bcl-2 protein.
25μg
Verified Reactivity: Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: N-terminal, Amino acid residues 61-76 of mouse Bcl-2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue4, immunocytochemical staining5, and immunoprecipitation5.
Application References(PubMed link indicates BioLegend citation): Hsu YT, et al. 1997. J. Biol. Chem. 272:13829.Zuber J, et al. 2011. Genes Dev. 25:1628. (WB) PubMedDoi K, et al. 2012. Biochem Biophys Res Commun. 425:107. PubMedYamanaka, et al. 2003. J. Immunol. 170:816. (IHC)Conus S, et al. 2000. EMBO J. 19:1534. (WB, IP, ICC)
Product Citations: Grebinoski S, et al. 2022. Nat Immunol. 23:868. PubMed Shallberg LA, et al. 2022. PLoS Pathog. 18:e1010296. PubMed VanDyke D, et al. 2022. Cell Rep. 41:111478. PubMed Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed Nakamura M, et al. 2015. J Immunol. 194:243. PubMed Bauché D et al. 2018. Immunity. 49(2):342-352 . PubMed Su W, et al. 2020. Cell Metabolism. 32(6):996-1011.e7. PubMed Wiede F, et al. 2021. Cancer Discov. Online ahead of print. PubMed Meyer LK, et al. 2019. J Clin Invest. 130:863. PubMed Yang HL, et al. 2020. Am J Reprod Immunol. 84:e13261. PubMed Beus J, et al. 2013. PLoS One. 8:71221. PubMed Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed Zhu X et al. 2019. Cell reports. 27(4):1205-1220 . PubMed Severance AL, et al. 2022. iScience. 25:104400. PubMed Huang H, et al. 2021. Cell. 184(5):1245-1261.e21. PubMed Zhang H, et al. 2021. Cell Reports. 35(6):109096. PubMed Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed Sun Y, et al. 2020. J Immunol. 205:2649. PubMed Wang H, et al. 2016. PLoS Pathog. 12: 1005787. PubMed Jin J, et al. 2020. Cell Reports. 30(12):4124-4136. PubMed
RRID: AB_2064149 (BioLegend Cat. No. 633509) AB_2274702 (BioLegend Cat. No. 633510)
Structure: Apoptosis regulator proteins, Bcl-2 family, BH1-4 domains. Homo- or hetero-dimer; isoforms α, β 25 kD, 22 kD
Distribution: Outer mitochondrial membrane, intracellular membrane nuclear envelope, endoplasmic reticulum
Function: Blocks apoptotic death by controlling the mitochondrial membrane permeability. Converted to pro-apoptotic activity by cleavage of BH4 domain. Regulates cell cycle progression via ROS
Modification: Phosphorylation, Ubiquitination
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
Antigen References: 1. Tsujimoto Y, et al. 1986 P. Natl. Acad. Sci. USA 83:5214. 2. Yang E, et al. 1995. Cell 80:285. 3. Huang Z, et al. 2000. Oncogene 19:6627. 4. Deng X, et al. 2003. Blood. 102:3179.
Regulation: Phosphorylation by ASK1/JNK1, PKC, ERKs, stress-activated kinases
Gene ID: 596
UniProt: View information about Bcl-2 on UniProt.org
Clone: BCL/10C4
Regulatory Status: RUO
Other Names: B-cell leukemia 2
Isotype: Mouse IgG1, κ