Biolegend, 633603, Alexa Fluor® 488 anti-Bax Antibody, 25μg

Bax is a 21 kD pro-apoptotic protein known to regulate apoptosis. Bax is found in the cytoplasm, mitochondria, and nucleus and is highly expressed in hematopoietic stem cells, ovaries, and lymph nodes. Bax binds the anti-apoptotic protein Bcl-2 as a heterodimer or forms homodimers. The relative levels of pro-apoptotic proteins such as Bax and anti-apoptotic proteins such as Bcl-2 determines whether cell death will occur following an apoptotic stimulus. Bax accelerates the opening of mitochondrial VDAC altering membrane potential and allowing cytochrome c to pass out of the mitochondria into the cytosol to initiate downstream caspase activation. p53 can transcriptionally activate the Bax gene to induce apoptosis. Bax has been shown to be mutated in some human cancers.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Amino acids 3-16 of human Bax protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone 2D2 has been shown to be useful for Western blotting1, immunoprecipitation2, immunofluorescence3 and immunohistochemical staining3 of formalin-fixed and paraffin-embedded tissue sections. This antibody does not cross-react with Bcl-2 or Bcl-XL proteins.Clone 2D2 does not show specificity for mouse BAX.
Application References(PubMed link indicates BioLegend citation): Hsu YT, et al. 1997. J. Biol. Chem. 272:13829. (WB) Cartron PF, et al. 2004. FEBS Lett. 578:41. (IP) Pucci S, et al. 2009. Cell Cycle 8:473. (IF, IHC)
Product Citations: Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed Yang HL, et al. 2020. Am J Reprod Immunol. 84:e13261. PubMed Lin J, Sorger M 2015. Sci Rep. 6: 8390. PubMed Herrera FG, et al. 2019. Int J Radiat Oncol Biol Phys. 103:320. PubMed
RRID: AB_2562171 (BioLegend Cat. No. 633603) AB_2562172 (BioLegend Cat. No. 633604)
Structure: Forms homodimers and heterodimers with Bcl-2, approximately 21 kD
Distribution: Cytoplasm, mitochondria, nucleus; expressed in hematopoietic stem cells, ovaries, lymph nodes
Function: Induces cell death through the process of apoptosis by acting on mitochondria
Interaction: 14-3-3 theta, VDAC1, Bcl-2, solute carrier family 25, mitochondrial carrier member 4
Cell Type: Hematopoietic stem and progenitors
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Antigen References: 1. LeBlanc H, et al. 2002. Nat. Med. 8:274. 2. Marzo I, et al. 1998. Science 281:2027. 3. Miyashita T et al. 1995. Cell 80:293. 4. Oltvai ZN, et al. 1993. Cell 74:609.
Gene ID: 581
UniProt: View information about Bax on UniProt.org
Clone: 2D2
Regulatory Status: RUO
Other Names: BCL2 associated X protein, apoptosis regulator Bax
Isotype: Mouse IgG1, κ