Biolegend, 633801, Purified anti-Bax Antibody, 25μg

Bax is a 21 kD pro-apoptotic protein known to regulate apoptosis. Bax is found in the cytoplasm, mitochondria, and nucleus and is highly expressed in hematopoietic stem cells, the ovary, and in the lymph node. Bax binds the anti-apoptotic protein Bcl-2 as a heterodimer or forms homodimers. The relative levels of pro-apoptotic proteins such as Bax and anti-apoptotic proteins such as Bcl-2 determines whether cell death will occur following an apoptotic stimulus. Bax accelerates the opening of mitochondrial VDAC altering membrane potential and allowing cytochrome c to pass out of the mitochondria into the cytosol to initiate downstream caspase activation. p53 can transcriptionally activate the Bax gene to induce apoptosis. Bax has been shown to be mutated in some human cancers. Clone 2D2 has been shown to be useful for western blotting, immunoprecipitation, immunofluorescence and immunohistochemistry of the human Bax protein.This antibody does not cross-react with Bcl-2 or Bcl-X L proteins.
25μg
Verified Reactivity: Human, Mouse, Rat, Monkey
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Amino acid:12-24 common to human, mouse, and rat Bax protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted at between 2°C and 8°C.
Application: IP - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunoprecipitation. This antibody can be used at 2-4 µg /1 x107 cell equivalents for immunoprecipitation. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Hsu YT, et al. 1997. J. Biol. Chem. 272:13829. Liu G, et al. 2013. J. Immunol. 191:2680. PubMed
Product Citations: Oda SK, et al. 2020. J Exp Med. 217: . PubMed Liu G, et al. 2013. J Immunol. 191:2680. PubMed
RRID: AB_2227699 (BioLegend Cat. No. 633801) AB_2061543 (BioLegend Cat. No. 633802)
Structure: Forms homodimers and heterodimers with Bcl-2, approximately 21 kD
Distribution: Cytoplasm, mitochondria, nucleus. Expressed in hematopoietic stem cells, ovary, lymph node
Function: Induces cell death through the process of apoptosis by acting on mitochondria.
Interaction: 14-3-3 theta, VDAC1, Bcl-2, solute carrier family 25, mitochondrial carrier member 4
Cell Type: Hematopoietic stem and progenitors
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
Antigen References: 1. LeBlanc H, et al. 2002. Nat. Med. 8:274. 2. Marzo I, et al. 1998. Science 281:2027. 3. Miyashita T et al. 1995. Cell 80:293. 4. Oltvai ZN, et al. 1993. Cell 74:609.
Gene ID: 581
UniProt: View information about Bax on UniProt.org
Clone: 6A7
Regulatory Status: RUO
Other Names: BCL2 associated X protein, apoptosis regulator Bax
Isotype: Mouse IgG1, κ