Biolegend, 633901, Purified anti-Bcl-X, 25μg

Bcl-X belongs to Bcl-2 family, and has several alternative splicing isoforms. It plays a critical role in control of apoptosis. The large isoform Bcl-XL (26kD) contains the highly conserved BH1/BH2 domain and protects cells from apoptosis, whereas the short isoform, Bcl-XS (19kD), which lacks of conserved BH1/BH2 domain, promotes apoptosis by interacting with Bcl-XL and Bcl-2. Bcl-XS is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-XL is found in long-lived, post-mitotic cells, such as adult brain.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Bcl-XS/L (3-14 amino acid, conserved in both human and mouse)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted at between 2°C and 8°C.
Application: WB - Quality tested IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Hsu YT, et al. 1997. J. Biol. Chem. 272:13829.
Product Citations: Kim HY, et al. 2014. Nat Med. 54:20. PubMed Hollenbaugh JA, et al. 2016. Virology. 106:491. PubMed Wang F, et al. 2020. Int J Oncol. 57:707. PubMed Inao T, et al. 2018. Oncotarget. 144:318. PubMed
RRID: AB_2064708 (BioLegend Cat. No. 633901) AB_2064706 (BioLegend Cat. No. 633902)
Structure: Five alternative splicing isoforms: Bcl-XS, Bcl-XL, Bcl-Xβ, Bcl-X, Bcl-XδTM
Distribution: Bcl-XS is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-XL is found in long-lived, post-mitotic cells, such as adult brain.
Function: Bcl-X plays a critical role in the control of apoptosis. The large isoform Bcl-XL (26kD) contains the highly conserved BH1/BH2 domain and protects cells from apoptosis, whereas the short isoform, Bcl-XS (19kD), which lacks of conserved BH1/BH2 domain, promotes apoptosis by interacting with Bcl-XL and Bcl-2.
Interaction: Bcl-2, Cytochrome C, JNK1, BAG 1, Caspase 8, Caspase 9, c-FLIP, Bax, p53
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
Antigen References: 1. Boise LH, et al. 1993 Cell 74:597
Gene ID: 598
UniProt: View information about Bcl-XS/L on UniProt.org
Clone: 2H12
Regulatory Status: RUO
Other Names: Apoptosis regulator Bcl-X, Bcl-2-like 1 protein
Isotype: Mouse IgG2a, κ