Biolegend, 634251, Purified anti-TATA Binding Protein Antibody, 25μg

The TATA-binding protein, also known as TBP or Tata Binding Protein, is the general transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), which plays a role in the activation of eukaryotic genes transcribed by RNA polymerase II. This protein is a component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC during RNA polymerase I-dependent transcription. The rate of PIC formation is assumed to be primarily dependent on the rate of the association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human TATA Binding Protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IP, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For the use of this antibody (clone W22002D) in immunocytochemistry (ICC), we recommend fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with 100% ice-cold methanol. This product does not cross-react with mouse or rat. This product is not suitable for use in immunohistochemistry on formalin-fixed paraffin-embedded tissues.
RRID: AB_3106159 (BioLegend Cat. No. 634251) AB_3106159 (BioLegend Cat. No. 634252)
Structure: TBP is composed of 339 amino acids with a predicted molecular weight of 37 kD.
Distribution: Nucleus
Function: General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II.
Interaction: Binds DNA as monomer. Belongs to the TFIID complex together with the TBP-associated factors (TAFs). Part of a TFIID-containing RNA polymerase II pre-initiation complex that is composed of TBP and at least GTF2A1, GTF2A2, GTF2E1, GTF2E2, GTF2F1, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2B, TCEA1, ERCC2, ERCC3, TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. Interacts with TFIIB, NCOA6, DRAP1, DR1 and ELF3, SPIB, SNAPC1, SNAPC2 and SNAPC4, UTF1, BRF2, UBTF, GPBP1, HIV-1 Tat, herpes simplex virus 1 ICP4, TBP-TATA complex, LLPH, HSF1.
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: Hoffmann A, et al. 1990. Nature. 346:387-90. Peterson MG, et al. 1990. Science. 248:1625-30. Kwon H, et al. 1994. J Biol Chem. 269:30140-6. Comai L, et al. 1994. Science. 266:1966-72.
Gene ID: 6908
UniProt: View information about TATA on UniProt.org
Clone: W22002D
Regulatory Status: RUO
Other Names: TATA-box-binding protein, TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID TBP subunit, TBP
Isotype: Rat IgG2a, κ