Biolegend, 634504, Alexa Fluor® 594 anti-Alix Antibody, 100μg

Alix (ALG-2-interacting protein X) is an adaptor protein that was first described for its capacity to bind to the calcium-binding protein ALG-2, the expression of which seemed necessary for cell death. The predicted molecular weight is approximately 96 kD. Alix contains an N-terminal Bro1 domain and a C-terminal proline-rich domain (PRD). The PRD contains multiple polyproline motifs, which are potential docking sites for proteins containing SH3 domains, interact with multiple cellular Alix-binding partners that are involved in apoptotic induction, endosomal sorting, and endocytosis. The Bro1 domain interacts with CHMP4b, which is also involved in endosomal sorting.
100μg
Verified Reactivity: Human, Mouse
Reported Reactivity: Rat, Frog
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant full-length human Alix protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Application Notes: Clone 3A9 has been shown to be useful for Western blotting1, immunoprecipitation, and immunofluorescence2 staining of the human and mouse Alix protein. Clone 3A9 shows strong specificity for mouse Alix.
Application References(PubMed link indicates BioLegend citation): Morel L, et al. 2013. J Biol Chem. 288:7105. (WB) PubMed Pan S, et al. 2008. EMBO J. 27:2077. (IF) Ertunc ME, et al. 2015. J Lipid Res. 56:423. PubMed
Product Citations: Salybekov AA, et al. 2021. Front Cardiovasc Med. 8:754254. PubMed
RRID: AB_2565615 (BioLegend Cat. No. 634504)
Structure: Belongs to a conserved family of proteins that have in common an N-terminal Bro1 domain and a C-terminal proline-rich domain. Predicted molecular weight approximately 96 kD.
Distribution: Cytoplasm. Alix is also abundant within extracellular vesicles known as exosomes, and enriched in virions that are released from cells under the direction of the HIV-1 Gag protein.
Function: Adaptor in signal transduction pathways. Involved in apoptosis and endosomal membrane trafficking and viral budding.
Interaction: ALG2, SH3GL2, H-Vps28, CHMP4B, CHMP4A, CHMP4C, SETA, CIN85, Src
Biology Area: Cell Biology
Antigen References: 1. Wu Y, et al. 2000. Differentiation 67:139. 2. Missotten M, et al. 1999. Cell Death Differ. 6:124. 3. Odorizzi G. 2006. J Cell Sci. 119:3025.
Gene ID: 10015
UniProt: View information about Alix on UniProt.org
Clone: 3A9
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: PDCD6IP, PDCD6 interacting protein, AIP1, Hp95
Isotype: Mouse IgG1, κ