Biolegend, 634602, Purified anti-hnRNPK Antibody, 100μg

hnRNP K (Heterogeneous nuclear ribonucleoprotein K) is a pre-mRNA binding protein and a component of hnRNP complexes providing substrate for pre-mRNA processing before becoming translatable mRNAs in cytoplasm. This protein containing KH domains and is ubiquitously expressed in the cytoplasm and the nucleus with a predicted molecular weight approximately 51 kD. hnRNP K expression and function are increased by epidermal growth factor (EGF). hnRNP K acts as transcriptional regulator and functions in nucleocytoplasmic shuttling, and is modified by phosphorylation (PKC δ, c-Src) and acetylation. Clone F45P9C7 has been shown to be useful for western blotting and immunohistochemistry of human, mouse, rat, rabbit and chicken hnRNP K.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Ovalbumin-conjugated synthetic Peptide SVKQYSGKFF
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Carpenter B, et al. 2006. Br J Cancer. 95:921. PubMed Carpenter B, et al. 2007. Br J Cancer. 97:574. PubMed Alnabulsi A, et al. 2009. Methods of cancer diagnosis, therapy and diagnosis. 4:25.
Product Citations: Carpenter B, et al. 2006. Br J Cancer. 95:921. PubMed Roychoudhury P, Chaudhuri K 2007. Br J Cancer. 97:574. PubMed
RRID: AB_2120365 (BioLegend Cat. No. 634601) AB_2120361 (BioLegend Cat. No. 634602)
Structure: Heterogenous nuclear ribonucleoprotein family, KH domains; 51 kD
Distribution: Ubiquitously expressed cytoplasmic and nuclear protein
Function: Pre-mRNA binding protein, component of hnRNP complexes providing substrate for pre-mRNA processing before becoming translatable mRNAs in cytoplasm. Acts as transcriptional regulator and functions in nucleocytoplasmic shuttling
Interaction: Interacts with Csk, NCK2, Vav1, ITK, CEBP β, c-Src, Fyn, Lyn, Hepatitis virus core protein
Modification: Phosphorylation (PKC δ, c-Src), Acetylation, Methylation
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Matunis MJ, et al. 1992. Mol. Cell. Biol. 12:164. 2. Dejgaard K, et al. 1994. J. Mol. Biol. 236:33. 3. Mandal M, et al. 2001. J. Biol. Chem. 276:9699
Regulation: Epidermal growth factor (EGF) can increase expression and function
Gene ID: 3190
UniProt: View information about hnRNPK on UniProt.org
Clone: F45P9C7
Regulatory Status: RUO
Other Names: Heterogeneous nuclear ribonucleoprotein K, DC-stretch binding protein, Transformation upregulated protein (TUNP), hnRNP K, hnRNP-K
Isotype: Mouse IgG1, κ