Biolegend, 634851, Purified anti-MDM2 Antibody, 25μg

MDM2 (E3 ubiquitin-protein ligase MDM2) serves a pivotal role in cellular regulation by acting as a negative regulator of the tumor suppressor protein p53, a key player in safeguarding genomic integrity. MDM2 regulates p53 in two ways. First, it negatively regulates p53 by directly binding to the transcriptional activation domain and preventing p53 from interacting with DNA. Second, MDM2 functions as an E3 ubiquitin ligase to target p53 for degradation via the proteasome. By negatively regulating p53, MDM2 prevents unnecessary activation of p53-mediated cellular responses such as cell cycle arrest, DNA repair, and apoptosis. MDM2 exists in a negative feedback loop with p53, as elevated levels of p53 induce transcription of MDM2 which in turn inhibits p53 function and promotes degradation. Dysregulation of MDM2 leads to disruption of the MDM2/p53 balance and can contribute to the development of various cancers, emphasizing the importance of understanding the role of MDM2 in cancer research. Furthermore, MDM2 has functions outside p53 regulation such as chromatin regulation, complicating attempts to target the protein in cancer therapy.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of the N-terminus of human MDM2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested ICC, WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 5 - 10 μg/mL is recommended. For western blotting, the suggested use of this reagent is 0.5 - 1 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For immunohistochemistry (IHC), we recommend antigen retrieval with either Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0) or Sodium Citrate H.I.E.R. (Cat. No. 928502). For human kidney tissue, we do not recommend using Tris-EDTA. For immunocytochemistry (ICC), we recommend fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with either 1) 0.5% Triton-X or 2) 100% ice-cold methanol. Fixation with 100% ice-cold methanol alone is not recommended.
RRID: AB_3106147 (BioLegend Cat. No. 634851) AB_3106147 (BioLegend Cat. No. 634852)
Structure: MDM2 is a 491 amino acid protein with a predicted molecular weight of 55.2 kD.
Distribution: Primarily nuclear
Function: Ubiquitin ligase, oncogene
Interaction: p53, HIPK2, p14arf, USP7
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Death, Cell Proliferation and Viability
Antigen References: Wade M, et al. 2013. Nat Rev Cancer. 13:83-96. Koo N, et al. 2022. Int J Mol Sci. 23(9):5005. Haupt Y, et al. 1997. Nature. 387:296-9. Wienken M, et al. 2017. J Mol Cell Biol. 9:74-80.
Gene ID: 4193
UniProt: View information about MDM2 on UniProt.org
Clone: W23003F
Regulatory Status: RUO
Other Names: Mouse double minute 2 homolog (MDM2), E3 ubiquitin-protein ligase MDM2, RING-type E3 ubiquitin transferase MDM2, Q00987
Isotype: Rat IgG2b, κ