Biolegend, 635051, Purified anti-ME2 Antibody, 25μg

ME2, also known as malic enzyme 2, is a mitochondrial NAD- or NADP-dependent enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate, involving the tricarboxylic acid (TCA) cycle for energy production. During the conversion of malate to pyruvate, ME2 also reduces NAD+ to NADH, participating in cellular respiration and serving as an electron carrier in the electron transport chain. It is a heteromeric protein that plays a crucial role in energy metabolism, cancer, cardiovascular disease, and neurological disorders. ME2 has been found to be upregulated in various types of cancer, including breast, lung, and liver cancer, promoting tumor growth and survival by providing cancer cells with the necessary energy and building blocks for rapid proliferation. Additionally, ME2 assists in glutaminolysis, providing another energy source for cancer cell proliferation. Moreover, P53 has been found to lower the expression of ME2, which induces ROS levels and decreases ATP production, preventing cellular senescence of tumor cells. Furthermore, there may be a link between ME2 and family-associated genetic generalized epilepsy.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human ME2 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone W18113C is not recommended for WB and IHC-P. For Immunocytochemistry (ICC), ice-cold methanol is recommended for the fixation and permeabilization method.
RRID: AB_3106145 (BioLegend Cat. No. 635051) AB_3106145 (BioLegend Cat. No. 635052)
Structure: ME2 has two isoforms due to alternative splicing.
Distribution: Wide tissue expression/mitochondrion matrix
Function: Catalyzes the oxidative decarboxylation of malate to pyruvate/regulate TCA cycle
Interaction: Form homodimer and homotetramer
Cell Type: Leukemia, Neurons, Osteoblasts
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Proliferation and Viability, Mitochondrial Function, Neuroscience, Synaptic Biology
Molecular Family: Enzymes and Regulators, Mitochondrial Markers, Organelle Markers
Antigen References: Pongratz R, et al. 2007. J Biol Chem. 282:200-7. MacDonald M, et al. 2009. Arch Biochem Biophys. 488:100-4. Wen Y, et al. 2014. Acta Pharmacol Sin. 35:674-84. Ren J, et al. 2014. Sci Rep. 4:5414. Wang Y, et al. 2021. Cell Metab. 33:1027-1041. Hsieh J, et al. 2023. Commun Biol. 6:548. Li W, et al. 2023. Proc Natl Acad Sci U S A. 120:e2217869120
Gene ID: 4200
UniProt: View information about ME2 on UniProt.org
Clone: W18113C
Regulatory Status: RUO
Other Names: Malic Enzyme 2; NAD-Dependent Malic Enzyme, Mitochondrial; Malate Dehydrogenase (Oxaloacetate-Decarboxylating); Malic Enzyme 2, NAD(+)-Dependent, Mitochondrial; NAD-ME; Pyruvic-Malic Carboxylase
Isotype: Rat IgG2a, κ