Biolegend, 635152, Purified anti-ZNF451 Antibody, 100μg

ZNF451, or Zinc finger protein 451, exhibits E3 SUMO ligase activity along with zinc finger domains that regulate biological processes. These processes include sumoylation, TGF-β signaling, and the stability of promyelocytic leukemia (PML) bodies within the nucleus. ZNF451 contains sumo-related domains which enables SUMO-protein ligase activity and interaction with SUMO 2. In mouse models, the sumoylation of PML proteins by ZNF451 decreases the stability of PML bodies. In addition, in response to cellular stressors such as topoisomerase 2 (TOP2) activity and DNA damage, ZNF451 binds to TOP2 leading to the recruitment of TDP2. This recruitment facilitates the unloading of the TOP2 cleavage complex to regulate DNA repair mechanisms. The zinc finger domains of ZNF451 effectively bind to SMAD2/3/4 proteins and attenuate upstream TGF-β signals. This interaction results in the repression of transcriptional activity, thereby influencing downstream cellular processes.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human ZNF451
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody (clone W19095A) is not suitable for immunocytochemistry (ICC). For immunohistochemistry (IHC), the recommended antigen retrieval buffer is either Tris-EDTA pH 9.0 (10 mM Tris, 1 mM EDTA) or Citrate Buffer, 10X (Cat. No. 420902).
RRID: AB_3106131 (BioLegend Cat. No. 635151) AB_3106131 (BioLegend Cat. No. 635152)
Structure: Forms a homooligomer
Distribution: Nuclear (promyelocytic leukemia body or nucleoplasm)
Function: Sumoylation, DNA repair
Interaction: Interacts with SUMO1, SUMO2, UBE2I/UBC9, SMAD2, SMAD3, SMAD4, ubiquitin, and SIMC1
Cell Type: Embryonic Stem Cells, Leukemia
Biology Area: DNA Repair/Replication, Signal Transduction, Transcription Factors, Ubiquitin/Protein Degradation
Molecular Family: Ligases
Antigen References: Karvonen U, et al. 2008. J Mol Biol. 382:585-600. Feng Y, et al. 2014. J Biol Chem. 289:2072-83. Cappadocia L, et al. 2015. Nat Struct Mol Biol. 22:968-75. Koidl S, et al. 2016. Int J Biochem Cell Biol. 79:478-487. Schellenberg MJ, et al. 2017. Science. 357:1412-1416.
Gene ID: 26036
UniProt: View information about ZNF451 on UniProt.org
Clone: W19095A
Regulatory Status: RUO
Other Names: ZATT, Zinc finger protein 451, Coactivator for steroid receptors, COASTER, E3 SUMO-protein transferase ZNF451, E3 SUMO-protein ligase ZNF451, Zinc Finger Protein Associated With TDP2 And TOP2
Isotype: Rat IgG2a, κ