Biolegend, 635301, Purified anti-TIMP-1 Antibody, 25μg

TIMP-1 (Tissue inhibitor of metalloproteinase 1) is a glycoprotein that belongs to the protease inhibitor I35 (TIMP) family. Contains 1 NTR domain, predicted molecular weight 23 kD. TIMP-1 forms complexes with matrix metalloproteinases (MMPs) and inhibits the proteolytic activity of these enzymes. High levels TIMP-1 protein has been found in several types of cancers including breast cancer and this increase associated with a poor patient prognosis. TIMP-1 has also been implicated in the inhibition of apoptosis, promotion of growth, and process of angiogenesis. TIMP-1 may be useful as a prognostic marker in combination with urokinase plasminogen activator (uPA) and plasminogen activator inhibitor type-1 (PAI-1). Clone F31P2A5 has been shown to be useful for western blotting, immunohistochemistry and ELISA of human and monkey TIMP-1.
25μg
Verified Reactivity: Human
Reported Reactivity: Non-Human Primate
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Ovalbumin-conjugated synthetic Peptide FQALGDAADIR
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ELISA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 4 µg per 1 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Murray G et al. 1998. Gut. 43:791. (IHC)
RRID: AB_2204557 (BioLegend Cat. No. 635302)
Structure: Belongs to the protease inhibitor I35 (TIMP) family. Contains 1 NTR domain, predicted molecular weight 23 kD.
Distribution: Extracellular, expressed in a number of connective tissues, plasma and amniotic fluid.
Function: TIMP-1 forms complexes with matrix metalloproteinases (MMPs) and inhibits the proteolytic activity of these enzymes. High levels of TIMP-1 protein has been found in several types of cancers (eg. breast cancer) where the increase has been associated with a poor patient prognosis.
Interaction: MMP-1, MMP-3, MMP-9
Biology Area: Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology
Molecular Family: Enzymes and Regulators
Antigen References: 1. Lambert E, et al. 2004. Crit. Rev. Oncol./Hematol. 49:187
Gene ID: 7076
UniProt: View information about TIMP-1 on UniProt.org
Clone: F31P2A5
Regulatory Status: RUO
Other Names: TIMP1, EPA, Collagenase inhibitor, Fibroblast collagenase inhibitor
Isotype: Mouse IgG1, κ